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通过光显微镜测量虫体pH值

Gerone A Gonzales1, Johnathan Canton2,3

  • 1Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.

Methods in molecular biology (Clifton, N.J.)
|June 26, 2023
PubMed
概括
此摘要是机器生成的。

双波长,双光体比度成像使有机体中活细胞pH值的动态研究成为可能. 这种先进的显微镜技术克服了用于精确细胞内pH值测量的其他方法的局限性.

关键词:
塞浦路斯人5e5e复合光比成像成像 复合光比成像双波长比率成像技术的双波长比率成像在光中光.光显微镜的光学显微镜.俄勒冈 绿色 绿色 俄勒冈 绿色发酵细胞体的发酵细胞体.在SNARF1中.它们的pH值为pH.的pHrodo 值.

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科学领域:

  • 细胞生物学 细胞生物学
  • 显微镜的使用方法
  • 生物物理学的生物物理.

背景情况:

  • 放射性成像使用双波长和光体来准确测量活细胞中的pH值.
  • 它纠正探头度和光漂白的变化,提高数据可靠性.
  • 这种技术与全种群方法相比,提供了更高的分辨率,使单细胞和器官分析成为可能.

研究的目的:

  • 详细介绍双波长,双光子比度成像的原理.
  • 解释其在测量体pH值中的应用.
  • 引导研究人员进行探头选择,仪器仪表和校准.

主要方法:

  • 使用双波长和双光体显微镜.
  • 应用比度分析来纠正实验变化.
  • 专注于细胞内体的活细胞成像,特别是体.

主要成果:

  • 证明比度成像对于活细胞中的动态pH监测的能力.
  • 考虑到焦平面的移动,探头加载和光漂白.
  • 已成功应用于体pH测量.

结论:

  • 双波长,双光体比度成像是细胞内pH值研究的一种强有力的方法.
  • 该技术为单个细胞组件提供了高分辨率.
  • 提供了关于实施的详细指导,包括探头的选择和校准.