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Updated: Jul 25, 2025

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
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一个体外系统来分析发育和降解的Phagosomal酸盐酸

Andreas Jeschke1

  • 1Institute for Cell Biology, University of Bonn, Bonn, Germany. jeschke@uni-bonn.de.

Methods in molecular biology (Clifton, N.J.)
|June 26, 2023
PubMed
概括

这项研究提出了一种新方法,用于追踪酶酸盐 (PIPs) 在细胞成熟过程中. 对于研究病原体如何操纵细胞过程来说,了解细胞中的PIP动态至关重要.

关键词:
五个域名域名脂质酸酶是一种脂质酸酶.细胞 (phagocytes) 是一种食物细胞.发细胞症 (phagocytosis) 是一种致死细胞的发生.发酵细胞体的发酵细胞体.酸盐酸酸盐酸酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐类化物检测检测方法酸酸酸盐酸酸盐酸酸盐酸酸盐酸酸盐酸酸盐酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸的新陈代谢

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Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
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Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
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