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参与尼拉帕尔瓦塔体色塑性发展的长波素在尼拉帕尔瓦塔体色塑性

Jia-Bao Lu¹, Ze-Dong Li², Zhuang-Xin Ye¹

¹State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.

|June 26, 2023

概括

对于视力至关重要的奥普辛还调节了虫尼拉帕尔瓦塔 (Nilaparvata lugens) 的身体颜色. 沉默Nllw opsin引发了黑色化,揭示了视觉基因和昆虫形态之间的联系.

科学领域:

昆虫学昆虫学是一门学科.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

奥普辛是重要的视觉光色素,具有不同的光谱灵敏度.
除了视觉之外,opsins已经发展出非视觉功能,尽管研究有限.
由于基因重复和损失,昆虫素的多样性有所增加.

研究的目的:

识别和描述迁移性大米害虫Nilaparvata lugens中的素.
使用RNA干扰 (RNAi) 调查N. lugens opsins的功能作用.
探索与素功能相关的基因表达模式.

主要方法:

基因组和转录组分析以识别N. lugens.中的opsins.
通过RNA干扰 (RNAi),使特定的素基因沉默.
Illumina Novaseq 6000平台用于转录基因组测序.

主要成果:

发现了四种素:一种长敏感 (Nllw) 和三种紫外线敏感 (NlUV1/2,NlUV3-like).
在眼睛中高度表达的奥普辛;建议NlUV1/2重复.
沉默Nllw导致身体颜色的黑化,并改变了NlTH和NlaaNAT基因的表达.

结论:

Nllw opsin 调节了 N. lugens 中的皮层黑化,这是 opsins 在 Hemiptera 中的一个新功能.
显示视觉系统基因路径和昆虫的形态差异化之间的交叉对话.

关键词:

身体颜色身体颜色基因组基因组基因组基因组基因组梅拉尼化的过程尼拉帕尔瓦塔 (尼拉帕尔瓦塔) 装饰品这就是Opsin.它们是RNAiRNAi.转录组转录组就是一个转录组.

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