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多巴胺通过在发育过程中依赖于黑色素调节胆能波来调节视网膜时钟

Chaimaa Kinane^1,2, Hugo Calligaro^1,3, Antonin Jandot¹

¹Inserm, Stem Cell and Brain Research Institute U1208, Univ Lyon, Université Claude Bernard Lyon 1, 18 Avenue du Doyen Lépine, 69500, Bron, France.

|June 26, 2023

概括

多巴胺 (DA) 和黑色素调节视网膜生长的昼夜时钟. DA通过多巴胺基受体延长时钟周期,并与乙胆波相互作用,影响视网膜发育和光反应.

科学领域:

神经科学是一个神经科学.
时间生物学时间生物学
发展生物学发展生物学

背景情况:

哺乳动物视网膜拥有内在的昼夜时钟,控制生理功能,包括多巴胺 (DA) 释放.
DA对于视网膜发育,视觉信号和时钟重置至关重要,已知与表达黑色素细胞的相互作用.
之前的研究指出,成年黑色素淘汰赛小鼠改变了视网膜时钟周期,但其发育作用仍然不清楚.

研究的目的:

研究多巴胺和黑色素对视网膜昼夜钟成熟的影响.
阐明DA和黑色素在成长中的视网膜内生功能和光反应中的作用.

主要方法:

使用野生类型的Per2^Luc和黑色素淘汰赛 (Opn4^-/-:Per2^Luc) 小鼠在各种出生后发育阶段.
评估了视网膜扩展体中自维持昼夜节律的出现和特征.
操纵多巴胺水平和阻断胆能视网膜波,观察对昼夜时钟周期和光引起的相位变化的影响.

主要成果:

视网膜昼夜节律是独立于从出生后第5天开始的外部线索生成的,无论是野生类型的还是黑色素的淘汰赛小鼠.
多巴胺补充剂在第一个产后周通过D1和D2类受体延长了野生类型视网膜的昼夜时钟周期.
阻断胆固醇视网膜波缩短了该周期,并减少了光诱导的相位移,特别是在野生类型视网膜中.

结论:

多巴胺通过依赖于黑色素调节视网膜胆能波来调节核心分子时钟.
这项研究揭示了多巴胺和黑色素在视网膜时钟内源功能和光响应的发育调节中的新作用.

关键词:

乙烯基胆是一种循环节律是指循环节律的节奏.多巴胺是一种多巴胺.灯光灯光灯光灯光梅拉诺普辛是什么意思梅拉诺普辛是什么意思视网膜波是指视网膜中的波.

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