差异表达的陪伴基因显示出一种应激反应,这种应激反应是基底状体Cionaona中单向再生所需的
William R Jeffery1,2,3, Bo Li4,5, Mandy Ng6
1Department of Biology, University of Maryland, College Park, MD, 20742, USA. jeffery@umd.edu.
BMC biology
|June 26, 2023
概括
涉及HSP70陪伴基因的应激反应对于Ciona intestinalis的再生至关重要. 在远端碎片中诱导这种应激反应促进干细胞的激活和再生,为脊椎动物的再生限制提供了洞察力.
科学领域:
- 发展生物学 发展生物学
- 再生医学是一种再生医学.
- 分子生物学分子生物学
背景情况:
- 基底弦细胞的再生涉及干细胞和祖细胞的活动.
- 尽管含有干细胞,但Ciona intestinalis的远端碎片无法再生.
- 了解远端碎片的再生失败是释放再生潜力的关键.
研究的目的:
- 研究远端Ciona intestinalis碎片中再生失败背后的分子机制.
- 在再生过程中识别参与干细胞激活和祖细胞迁移的基因和途径.
- 确定应激反应途径在启动和支持再生中的作用.
主要方法:
- 转录基因组测序和再生Ciona intestinalis分支囊的组装.
- 权重基因相关性网络分析,以确定与再生相关的基因模块.
- 通过siRNA介导的基因敲除来评估基因在再生中的功能.
- 用热冲击治疗来研究应激反应诱导及其对再生的影响.
主要成果:
- 确定了1149个差异表达的基因,分为再生相关 (上调) 和代谢/恒常相关 (下调) 模块.
- HSP70陪伴系统基因 (hsp70,dnaJb4,bag3) 具有高度上调,对于祖细胞向和再生至关重要.
- 远端碎片缺乏应激反应,但热冲击诱导了hsp70/dnaJb4表达,细胞增殖,并促进了再生.
结论:
- 由HSP70陪伴基因介导的应激反应对于Ciona intestinalis的干细胞激活和再生至关重要.
- 远端碎片中缺乏这种应激反应解释了它们无法再生的原因.
- 诱导应激反应提供了一种促进再生的潜在策略,对脊椎动物有影响.
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