多种多样化的转子子谱系有助于早期和最近的双鱼基因组进化
Jacopo Martelossi1, Filippo Nicolini1,2, Simone Subacchi1
1Department of Biological Geological and Environmental Science, University of Bologna, Via Selmi 3, 40126, Bologna, Italy.
BMC biology
|June 26, 2023
概括
可转移元素,或TE,是真核生物中基因组变异的关键驱动因素. 这项研究揭示了双动物中异常的TE多样性,长间隔核元素 (LINE) 在它们的基因组进化和多样化中发挥着重要作用.
科学领域:
- 基因组学就是基因组学.
- 进化生物学 进化生物学
- 分子生物学分子生物学
背景情况:
- 可转移元素 (TE) 是真核生物中基因组变异和创新的主要来源.
- 软体动物,特别是双动物,在TE进化动态方面仍未得到充分研究.
- 软体动物的基因组资源最近得到了扩大,从而开辟了新的研究途径.
研究的目的:
- 在27个双体基因组中描述TE谱和进化动态.
- 专注于DDE/D II类元素和长间隔核元素 (LINE).
- 为全面分析利用自动注释和家族遗传分类.
主要方法:
- 自动化的TE注释管道.
- 基于家族遗传树的分类.
- 对 TE 数据进行了广泛的手动策划.
- 在27个两动物基因组的比较分析.
主要成果:
- 一类元素,特别是LINE,在两动物基因组中占主导地位,覆盖率高达10%.
- 确定了86,488个含逆转录酶的LINE和14,275个II类DDE/D转位子.
- 发现了一种丰富的祖先转位子补充物,可以追溯到共同祖先 (约500万年前).
- 观察到特定于谱系的LINE和DDE/D元素的出现和丧失,其中一些在双特定的谱系中得到放大.
- 发现了长寿,潜在活跃的LINE元素的证据,维持现存物种的多样性.
结论:
- 与其他软体动物相比,双动物表现出异常的转位子多样性.
- 双动物中的线性元素可能遵循"隐形驱动器"模型,并存并塑造基因组进化.
- 提供了第一个关于软体体体的TE动态的比较研究,以及LINE和DDE/D元素的参考库.
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