在LPS诱导的炎症微环境中,G-CSF对hPDLSC增殖和骨质分化的影响
Hui Yu1, Pengcheng Wang2, Haibin Lu1
1Department of Stomatology, Affiliated Zhongshan Hospital of Dalian University, 6th Jiefang Street, Dalian, Liaoning, China.
BMC oral health
|June 26, 2023
概括
颗粒细胞殖民地刺激因子 (G-CSF) 抑制人类牙周带干细胞 (hPDLSC) 的增殖和骨质分化. 这项研究发现hPDLSCs上的G-CSF受体 (G-CSFR) 表达,揭示了牙周炎相关的骨损失的机制.
科学领域:
- 干细胞生物学 干细胞生物学
- 牙周病学 牙周病学
- 骨生物学 骨生物学
背景情况:
- 牙周炎是一种慢性传染病,导致膜骨再吸收和牙损失.
- 颗粒细胞殖民地刺激因子 (G-CSF) 与牙周炎相关的骨再吸收有关.
- G-CSF对骨重塑的影响的确切机制,特别是涉及人类牙周带干细胞 (hPDLSCs),仍然不清楚.
研究的目的:
- 研究G-CSF在hPDLSC增殖和骨质分化的作用.
- 探索G-CSF对牙周组织修复的影响.
- 阐明G-CSF对牙周炎中异常骨改造的影响背后的机制.
主要方法:
- 人类牙周带干细胞 (hPDLSCs) 被培养和鉴定.
- 在hPDLSCs上的G-CSF受体 (G-CSFR) 表达式使用免疫光学分析.
- 使用CCK8,阿利沙林红色染色,骨质基因RT-PCR (ALP,Runx2,OCN) 和PI3K/Akt通路组件的西部斑点来评估G-CSF对hPDLSCs在脂多糖 (LPS) 诱导的炎症环境中的影响.
主要成果:
- 证实G-CSFR在hPDLSCs的细胞表面膜上表达.
- 在LPS诱导的炎症微环境中,G-CSF显著抑制了hPDLSC的增殖和骨质分化.
- G-CSF治疗减少了关键骨质生成相关基因的表达,并增加了PI3K和Akt的酸化.
结论:
- G-CSF受体 (G-CSFR) 在hPDLSCs上表达.
- 在LPS诱导的炎症环境中,G-CSF在体外抑制hPDLSC骨质分化.
- 这些发现表明,G-CSF通过影响hPDLSC功能,起到影响牙周再生的作用.
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