DNA甲基化和基因调节之间的关联取决于跨代可塑性期间的染色质可访问性
Samuel N Bogan1, Marie E Strader2,3, Gretchen E Hofmann2
1Department of Ecology, Evolution and Marine Biology, University of California Santa Barbara, Santa Barbara, USA. samuel.n.bogan@gmail.com.
BMC biology
|June 26, 2023
概括
环境条件可以改变DNA甲基化,影响基因表达和表型可塑性. 这项研究揭示了DNA甲基化.
科学领域:
- 表观遗传学和发育生物学
- 海洋生物学 海洋生物学
- 基因组学就是基因组学.
背景情况:
- 包括DNA甲基化在内的表观遗传过程与表型可塑性期间调节基因表达有关.
- 环境诱导的DNA甲基化变化与元动物中差异性基因表达的联系有限.
- 作为对环境因素的反应,DNA甲基化,基因表达和染色质可访问性之间的相互作用仍未得到充分探索.
研究的目的:
- 研究环境诱导的DNA甲基化和基因表达变化之间的关系.
- 探索染色体可访问性在调解DNA甲基化对基因表达和拼接的影响中的作用.
- 作为差异甲基化和染色质可访问性的函数,对母体条件的转录反应进行建模.
主要方法:
- 在紫色海 (Strongylocentrotus purpuratus) 幼虫中对DNA甲基化和基因表达的量化.
- 幼虫暴露于生态相关的条件在游戏生成 (母体调节) 期间.
- 基因表达和剪接变化的建模,使用差异甲基化,染色质可访问性和基因组特征作为共变量.
主要成果:
- 在差异性DNA甲基化,染色质可访问性和影响差异性基因表达和拼接的基因特征之间检测到显著的相互作用.
- 不同基因体甲基化对具有更难访问的转录起点的基因对基因表达有更强烈的影响.
- 考虑到DNA甲基化和染色质可访问性之间的相互作用,检测对母体条件的转录反应的可能性增加了4-13倍.
结论:
- 基因甲基化在海和其他潜在的甲基动物的跨代可塑性和基因调节中发挥着作用.
- DNA甲基化的调节效应取决于染色质的可访问性和潜在的遗传特征.
- 染色质状态部分解释了差异性DNA甲基化和基因调节之间的关系.
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