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从Codonopsis pilosula中分离出的内细胞的抗菌活性和酶分析

Rathna Silviya Lodi¹, Xiaodan Dong¹, Chunhui Jiang^1,2

¹Key Laboratory of Agro-Products Processing Technology of Shandong Province, Key Laboratory of Novel Food Resources Processing Ministry of Agriculture, Institute of Food and Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, 23788, Gongye NRd, Licheng district, Jinan, Shandong Province, 250100, China.

FEMS microbiology ecology

|June 27, 2023

概括

来自Codonopsis pilosula根的内植物对人类病原体具有显著的抗菌活性. 这些微生物还产生像粉酶和蛋白酶这样的酶,为生物基应用提供了潜力.

科学领域:

微生物学和生物技术
药用植物研究药用植物研究

背景情况:

科多诺普西斯 (Codonopsis pilosula) 根是一种药用化合物的来源.
药用植物内的内性微生物可以产生生物活性物质.

研究的目的:

从C. pilosula根中分离和识别内性细菌.
评估这些内生植物对人类病原体的抗菌潜力.
描述由有前途的内菌株产生的酶.

主要方法:

从C. pilosula根中分离和识别内性细菌.
使用标准测定进行抗菌活性查.
高性能液体染色学 (HPLC) 用于二次代谢物分析.
二甲基硫酸盐聚烯胺凝电泳 (SDS-PAGE) 用于酶分析.
确定酶活动的最佳pH值和温度.

主要成果:

两个内菌株CP-8和CP-20显示出显著的抗菌活性.
C.P-8产生了一种具有抗微生物特性的二次代谢物,其最小抑制度 (MIC) 为250μg/ml,用于对抗 Staphylococcus aureus.
C.P-20 产生了氨基酶 (64 kDa),蛋白酶 (64 kDa),基因酶 (30 kDa) 和细胞酶 (54 kDa).
来自CP-20的部分净化酶在pH 6-7和40-45°C的温度下表现出最佳活性.

结论:

来自C. pilosula根的内细胞是抗菌剂的宝贵来源.
这些内植物可以产生工业相关的酶.
对这些内植物的进一步研究可能会导致新的基于生物的抗微生物药物和酶.

关键词:

科多诺普西斯 (Codonopsis pilosula) 是一个的植物.抗微生物活性的抗微生物活性.末类植物是内类植物.酶活性的酶活性.二次代谢产物二次代谢产物

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