通过再组合性 Pseudomonas putida KT244040 的高效生物合成Protocatechuic 酸
Jin Li1, Jianli Fu1, Cheng Yue1
1Laboratory of Biosystems and Microanalysis, Institute of Engineering Biology and Health, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Journal of agricultural and food chemistry
|June 27, 2023
概括
这项研究设计了*Pseudomonas putida*来生物合成原卡特丘酸 (PCA),达到38.8g/L. 这种微生物工厂方法为天然酸生产提供了一个有希望的替代方案.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 微生物生产 微生物生产
背景情况:
- 植物衍生的酸,如原卡特丘酸 (PCA),具有显著的生理活动和市场潜力.
- 传统的PCA生产方法面临挑战,无法满足日益增长的需求.
- 开发高效的生物合成路径对于可持续的PCA供应至关重要.
研究的目的:
- 设计一个高效的微生物工厂用于PCA生物合成,使用*Pseudomonas putida* KT2440.
- 通过基因改造来增强对PCA生产的代谢流.
- 探索用于优化微生物系统中酶水平的新策略.
主要方法:
- 伪白菌的代谢工程 KT2440.
- 删除葡萄糖酸二脱酶基因以重定向葡萄糖代谢.
- 基因组插入 *aroG*, *aroQ* 和 *aroB* 基因的额外副本.
- 应用降解标签 (GSD和DAS) 来降低石基酸脱酶水平.
主要成果:
- 工程菌株KGVA04产生了7.2g/L的PCA.
- 使用降解标签的优化提高了PCA产量,达到13.2 g/L (摇瓶) 和38.8 g/L (食批).
- 在P. putida*中使用降解标签证明了蛋白质水平酶调节的成功.
结论:
- *P. putida*的代谢工程是PCA生物合成的有效策略.
- 降解标签的使用代表了微调酶丰度和提高产品产量的新方法.
- 这种方法对自然生产酸具有重大潜力.
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