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阅读器MHYTP2通过与MdRGA2LmRNA结合和降解它,从而负调节果格洛梅雷拉叶斑抗性

Tianli Guo¹, Ru Bao¹, Zehua Yang¹

¹State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, China.

Molecular plant pathology

|June 27, 2023

概括

马卢斯hupehensis YT521-B同源域含有蛋白2 (MhYTP2) 影响果对Glomerella叶斑 (GLS) 的抗性. 这项研究揭示了MhYTP2通过影响R基因稳定性来降低抗性,确定MdRGA2L作为关键的抗性基因.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

由*Colletotrichum fructicola*引起的GLS是果生产的主要威胁.
由抗性基因 (R基因) 编码的核酸结合部位和氨酸丰富的重复 (NBS-LRR) 蛋白质,介导植物疾病耐药性.
在果中赋予GLS耐药性的特定R基因尚不清楚.

研究的目的:

为了研究马卢斯hupehensis YT521-B同源域含有蛋白2 (MhYTP2) 在果抗 Glomerella 叶斑 (GLS) 的作用.
确定MhYTP2的功能是否依赖于N6甲基氨酸 (m6A) RNA的修饰.
为了确定涉及GLS耐药性的新型R基因.

主要方法:

对先前生成的RNA免疫沉降测序 (RIP-seq) 数据进行分析.
在果植物中MhYTP2的过度表达.
定量实时PCR (qRT-PCR) 用于测量R基因转录水平.
mRNA稳定性测试.mRNA稳定性测试.mRNA稳定性测试.mRNA稳定性测试.

主要成果:

在MHYTP2中,既有A-依赖的功能,也有A-独立的功能.
过度表达MhYTP2降低了果对GLS的抵抗力,并降低了缺乏m6A修改的特定R基因转录.
MhYTP2与MdRGA2LmRNA结合,降低了它的稳定性.
通过酸信号传递,MdRGA2L积极调节GLS电阻.

结论:

MhYTP2在调节果对GLS的耐药性方面发挥着至关重要的作用.
MdRGA2L被确定为一种有前途的R基因,用于开发抗GLS的果品种.
了解MHYTP2的调节机制,可以了解植物免疫中的m⁶A独立基因调节.

关键词:

马卢斯 (Malus) 是一个不良的东西.格洛梅雷拉的叶子斑点是什么?在R基因,R基因,R基因.YTH 域名域名盐酸盐酸是什么盐酸盐酸是什么

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