探索区分Lactobacillus jensenii和Lactobacillus mulieris的基因型和表型差异
Adriana Ene1, Swarnali Banerjee2, Alan J Wolfe3
1Bioinformatics Program, Loyola University Chicago , Chicago, Illinois, USA.
mSphere
|June 27, 2023
概括
这项研究使用基因组和代谢分析来区分Lactobacillus jensenii和Lactobacillus mulieris. 树脂的利用是L. jensenii的特点,它有助于泌尿器官微生物群的研究.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 泌尿器官微生物组研究
背景情况:
- 乳酸菌种在女性泌尿器官中占主导地位.
- 之前的基因组分析在L. jensenii和L. mulieris中发现了独特的基因.
- 需要对基因型和表型差异进行进一步的研究.
研究的目的:
- 扩大L. jensenii和L. mulieris. 的基因组数据.
- 分析这些物种之间的基因型和表型区别.
- 在微生物组研究中确定区分L. jensenii和L. mulieris的标记物.
主要方法:
- 扩展基因组测序到61个菌株.
- 进行了核心基因组遗传学.
- 进行生物合成基因集群和代谢途径分析.
- 在尿路菌株中测试的碳水化合物利用率 (麦芽糖,三糖,葡萄糖,核糖).
主要成果:
- L. jensenii使用麦芽糖,三糖和葡萄糖;L. mulieris使用麦芽糖和葡萄糖,但不使用三糖或糖.
- 代谢途径分析证实,L. mulieris.中没有三糖代谢基因 (treB).
- 短读16S rRNA基因测序不足以区分这些物种.
- 在基因型和尿路症状状态之间没有发现任何关联.
结论:
- 树脂糖的运输和利用是L. jensenii. 的关键特征.
- 建议进行元基因组测序或物种特异性基因测序,以准确识别.
- 已识别的标记物可以帮助在女性泌尿器官微生物群研究中区分L. jensenii和L. mulieris.
- 无论是物种还是特定的基因型都没有显示出与尿路症状的强烈关联.
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