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相关概念视频

NMR Spectroscopy Of Amines01:19

NMR Spectroscopy Of Amines

9.0K
In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
9.0K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

473
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
473
IR and UV–Vis Spectroscopy of Aldehydes and Ketones01:29

IR and UV–Vis Spectroscopy of Aldehydes and Ketones

5.8K
Infrared spectroscopy, also known as vibrational spectroscopy, is mainly used to determine the types of bonds and functional groups in molecules. In aldehydes and ketones, the carbonyl (C=O) bond shows an absorption around 1710 cm-1. The C=O bond vibration of an aldehyde occurs at lower frequencies than that of a ketone. In addition to the C=O absorption in an aldehyde, the aldehydic C–H bond also gives two peaks in the 2700–2800 cm-1 range. This absorption, coupled with the...
5.8K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

466
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
466
Spectroscopy of Carboxylic Acid Derivatives01:26

Spectroscopy of Carboxylic Acid Derivatives

2.5K
Infrared spectroscopy is primarily used to determine the types of bonds and functional groups. In carboxylic acid derivatives, a typical carbonyl bond absorption is observed around 1650–1850 cm−1. For esters, the absorption is recorded at around 1740 cm−1, while acid halides show the absorption at about 1800 cm−1. Another acid derivative, the acid anhydrides, exhibit two carbonyl absorption around 1760 cm−1 and 1820 cm−1, arising from the symmetrical and...
2.5K
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

1.1K
Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single...
1.1K

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相关实验视频

Updated: Jul 25, 2025

Fast and Accurate Exhaled Breath Ammonia Measurement
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拉曼光谱检测尿素呼吸试验

Evgeniy Popov1, Anton Polishchuk1, Anton Kovalev1

  • 1Institute of Advanced Data Transfer Systems, ITMO University, Birzhevaya Liniya 14, 199034 Saint Petersburg, Russia.

Biosensors
|June 27, 2023
PubMed
概括
此摘要是机器生成的。

这项研究引入了拉曼光谱气体分析仪,用于更准确的尿素呼吸测试,以检测Helicobacter pylori感染. 这种新方法实现了6%的总误差,改进了这种非侵入性诊断测试的现有技术.

关键词:
在Helicobacter Pylori的研究中,拉曼光谱法 拉曼光谱法 拉曼光谱法呼出的气息 呼出的气息尿素呼吸试验 尿素呼吸试验δ13CC 这种情况是什么?

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One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
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Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
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相关实验视频

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Fast and Accurate Exhaled Breath Ammonia Measurement
06:27

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One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
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Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
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科学领域:

  • 分析化学 分析化学
  • 医学诊断 医学诊断 医学诊断
  • 频谱学是一种光谱学.

背景情况:

  • 尿素呼吸试验是诊断Helicobacter pylori感染的一个关键的非侵入性方法.
  • 目前使用非分散性红外传感器的方法由于测量错误,其精度受到限制.
  • 拉曼光谱在检测出口空气中的CO2同位素比率方面提供了提高精度的潜力.

研究的目的:

  • 开发和评估一个基于拉曼散射的气体分析仪,用于精确地在呼气中测量 δ13C.
  • 为了评估这种新系统对尿素呼吸测试的准确性.
  • 为了解决影响Helicobacter pylori检测的测量不确定性.

主要方法:

  • 基于拉曼散射的气体分析仪的开发.
  • 测量标准气体样本以校准12CO2和13CO2.
  • 对呼出的空气拉曼光谱进行分析,以计算尿素呼吸试验期间的Δ13C变化.

主要成果:

  • 基于拉曼散射的气体分析仪证明了对δ13C测量的能力.
  • 确定了12CO2和13CO2的校准系数.
  • 该系统实现了6%的总测量误差,这在分析计算的10%的极限内.

结论:

  • 拉曼光谱为尿素呼吸测试提供了一个可行的,准确的替代方案.
  • 开发的气体分析仪提高了用于Helicobacter pylori诊断的CO2测量的精度.
  • 这一进步可以导致更可靠的非侵入性检测H. pylori感染.