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拉曼光谱检测尿素呼吸试验

Evgeniy Popov¹, Anton Polishchuk¹, Anton Kovalev¹

¹Institute of Advanced Data Transfer Systems, ITMO University, Birzhevaya Liniya 14, 199034 Saint Petersburg, Russia.

|June 27, 2023

概括

这项研究引入了拉曼光谱气体分析仪,用于更准确的尿素呼吸测试,以检测Helicobacter pylori感染. 这种新方法实现了6%的总误差,改进了这种非侵入性诊断测试的现有技术.

科学领域:

分析化学分析化学
医学诊断医学诊断医学诊断
频谱学是一种光谱学.

背景情况:

尿素呼吸试验是诊断Helicobacter pylori感染的一个关键的非侵入性方法.
目前使用非分散性红外传感器的方法由于测量错误,其精度受到限制.
拉曼光谱在检测出口空气中的CO2同位素比率方面提供了提高精度的潜力.

研究的目的:

开发和评估一个基于拉曼散射的气体分析仪,用于精确地在呼气中测量 δ13C.
为了评估这种新系统对尿素呼吸测试的准确性.
为了解决影响Helicobacter pylori检测的测量不确定性.

主要方法:

基于拉曼散射的气体分析仪的开发.
测量标准气体样本以校准12CO2和13CO2.
对呼出的空气拉曼光谱进行分析,以计算尿素呼吸试验期间的Δ13C变化.

主要成果:

基于拉曼散射的气体分析仪证明了对δ¹³C测量的能力.
确定了¹²CO₂和¹³CO₂的校准系数.
该系统实现了6%的总测量误差,这在分析计算的10%的极限内.

结论:

拉曼光谱为尿素呼吸测试提供了一个可行的,准确的替代方案.
开发的气体分析仪提高了用于Helicobacter pylori诊断的CO2测量的精度.
这一进步可以导致更可靠的非侵入性检测H. pylori感染.

关键词:

在Helicobacter Pylori的研究中,拉曼光谱法拉曼光谱法拉曼光谱法呼出的气息呼出的气息尿素呼吸试验尿素呼吸试验 δ13CC 这种情况是什么?

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