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细菌细菌的特征 S-16 醇合成相关基因 thiS 淘汰和抗菌活性分析

Jinghan Hu¹, Zhenhe Su², Baozhu Dong^1,3

¹College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010020, China.

Current issues in molecular biology

|June 27, 2023

概括

在 Bacillus subtilis S-16 中删除 thiazole-biosynthesis 基因 (thiS) 减少了 2-甲基二醇 (2-MBTH) 的产生. 这削弱了生物控制剂抑制Sclerotinia sclerotiorum生长和毒性的能力.

科学领域:

微生物学微生物学
植物病理学植物病理学
生物化学生化学

背景情况:

细菌细菌S-16是一种土壤细菌,产生具有抗真菌性能的挥发性有机化合物 (VOC).
2-甲基二 (2-MBTH) 是一种主要的抗真菌化合物,由B. subtilis S-16产生的,有效对抗Sclerotinia sclerotiorum.

研究的目的:

为了研究 thiazole-biosynthesis gene (thiS) 在 B. subtilis S-16.6 的 2-MBTH 生产中的作用.
评估S删除对B. subtilisS-16挥发性有机化合物对S. sclerotiorum的抗菌活性的影响.

主要方法:

通过同源重组的基因删除来创建一个THIS突变菌株.
气色谱-并联质谱 (GC-MS/MS) 用于量化2-MBTH.
双培养试验,扫描电子显微镜 (SEM) 和植物病变试验,以评估抗真菌功效和毒性.

主要成果:

与野生类型菌株相比,thiS删除突变产生的2-MBTH显著减少.
来自突变菌株的VOC显示S. sclerotiorum菌株生长的抑制降低,并改变了形态.
突变的挥发性有机物导致日叶损伤增加,并且S. sclerotiorum对硬化菌形成的抑制减少.

结论:

这种thiS基因对于B. subtilis S-16.16中的2-MBTH生物合成至关重要.
由于thiS删除而减少的2-MBTH产生,降低了B. subtilis S-16 VOCs对S. sclerotiorum的生物控制有效性.

关键词:

2 - 甲基索提亚二醇的使用这种细菌是 Bacillus subtilis.太阳花的斯克莱罗提尼亚.这个S是S的.挥发性有机化合物挥发性有机化合物

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