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指南针复合体通过菌病原体Cryptococcus neoformans中的基因组交叉调节真菌发育和毒性

Ruoyan Liu¹, Xiaoyu Chen¹, Fujie Zhao¹

¹College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.

Journal of fungi (Basel, Switzerland)

|June 27, 2023

概括

研究人员在真菌病原体Cryptococcus neoformans中确定了与Set1相关的蛋白质综合体 (COMPASS) 的核心子单元. 这种复合物对于组素甲基化至关重要,调节真菌发育和毒性.

科学领域:

分子生物学分子生物学
菌类学菌类学是指菌类学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

与Set1相关的蛋白质综合体 (COMPASS) 是一个参与基因组甲基化过程的保存表观遗传调节器.
它在*Cryptococcus neoformans*的真菌病原体中的功能和组成在很大程度上是未知的.
在*C. neoformans*中理解COMPASS对于破译真菌发育和病变产生至关重要.

研究的目的:

在 *Cryptococcus neoformans* 和 *C. deneoformans* 中确定 COMPASS 综合体的核心子单位.
阐明COMPASS在加密菌发育和毒性方面的监管作用.
为了研究*C. neoformans*中COMPASS介导的素甲基化机制.

主要方法:

亚单元识别和复杂组件分析.
对COMPASS子单元的AlphaFold蛋白质结构预测.
基因组甲基化试验和基因表达分析.
功能性测试用于加密球菌的发展和毒性.

主要成果:

在*C. neoformans*和*C. deneoformans*中发现并证实了核心COMPASS子单位 (Set1, Bre2, Swd1, Swd3).
阿尔法模型揭示了COMPASS复合体的催化核心.
COMPASS调节了酵母转化为菌的过程,以及C. neoformans*的耐热性和毒性.
通过 COMPASS 的基因组 H3K4 甲基化需要 H2B 单双化和 Paf1 复合体.

结论:

已识别的COMPASS子单位在*C. neoformans*中形成了一个功能复合体.
COMPASS在H3K4甲基化中起着保留作用,影响密码球菌的发展和毒性.
这项研究提供了关于真菌病原体的表观遗传调节的见解.

关键词:

一个指南针指南针.这是Cryptococcus neoformans的新型菌.H2B无处不在的存在在H3K4甲基化过程中.毒性毒性是一种毒性.酵母转化为菌的过渡过程

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