在真菌学应用中OMICS和其他先进技术
Nalin N Wijayawardene1,2,3, Nattawut Boonyuen4, Chathuranga B Ranaweera5
1Centre for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing 655011, China.
Journal of fungi (Basel, Switzerland)
|June 27, 2023
概括
像DNA测序和奥米克等先进的分子技术正在彻底改变真菌识别,特别是对于挑战"暗种群"而言. 这扩大了我们对真菌的理解.
科学领域:
- 菌类学和分子生物学 菌类学和分子生物学
- 生态系统动力学和真菌的作用
背景情况:
- 对于生态系统至关重要,但对于各种应用来说,精确的识别至关重要.
- 传统的形态识别是有限的,特别是对于缺乏明显特征的真菌 ("暗种群").
- 技术进步提供了改善的真菌识别和分类.
研究的目的:
- 讨论用于真菌识别的多种分类学方法.
- 突出奥米克技术在真菌研究中的重要性.
- 探索真菌知识在食品安全和医学中的应用.
主要方法:
- 聚合酶链反应 (PCR) 放大和核糖体DNA (rDNA) 的DNA测序.
- 多个位置的遗传学分析,以便进行可靠的分类.
- 应用各种omics技术:蛋白质组学,转录组学,代谢组学等.
主要成果:
- 先进的测序和元基因组学有助于识别新的真菌系,包括"暗种群".
- 欧米克技术为真菌生物学和功能提供了全面的理解.
- 这些方法对于扩大有关真菌影响和应用的知识至关重要.
结论:
- 现代分子和奥米克方法对于准确的真菌分类学和发现至关重要.
- 了解真菌是推动食品安全,医学和生物技术进步的关键.
- 在极端和研究不足的环境中对真菌的进一步探索对于识别新血统至关重要.
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