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亚氏氧化原性Aspergillus通过抗氧化机制调节亚氏氧-B1水平

Bwalya Katati^1,2, Stan Kovacs¹, Henry Njapau²

¹Laboratory of Genetics, Wageningen University and Research, 6708 PB Wageningen, The Netherlands.

Journal of fungi (Basel, Switzerland)

|June 27, 2023

概括

抗氧化性Aspergillus Flavi降解了阿弗拉托辛B1,而毒性菌株则将其用作抗氧化剂. 暴露有利于抗氧化菌株,这表明生物控制的潜力.

科学领域:

菌类学菌类学是指菌类学.
植物病理学植物病理学
生物化学生化学

背景情况:

由Aspergillus部分Flavi产生的亚毒素 (AFs) 已在真菌生物学中发挥作用,包括抗氧化和昆虫威.
众所周知,Flavi的亚氧化菌株会降解亚毒素B1 (B1),但这种降解的目的尚不清楚.

研究的目的:

调查亚毒素降解,特别是B1和G1 (G1) 在Flavi.的抗氧化能力中的作用.
确定抗氧化剂 (Se) 对 aflatoxin水平的影响以及毒性与无氧性Flavi菌株的适应性.

主要方法:

有毒和无毒的Flavi分离物被人工B1和G1化,有或没有.
使用高性能液态染色学 (HPLC) 量化了阿弗拉托克辛水平.
在暴露于不同度的Se在3%糖糖玉米粉 (3gCMA) 上后,通过子计数评估了真菌适应性.

主要成果:

氧化剂Flavi降低了B1水平,而G1水平在没有Se的情况下保持不变.
治疗导致毒性Flavi减少B1消化,并增加G1水平.
不影响B1消化在任何菌株类型的无氧化Flavi或G1水平.
在0.86μg/g Se.的Atoxigenic菌株表现出明显高于毒性菌株的适应性.

结论:

毒性Flavi通过抗氧化机制调节B1水平,在这个作用中更喜欢B1而不是G1.
氧化剂的Flavi可以积极降解B1.1.
在应激下,抗氧化菌株的增强适应性表明它们在抗毒性菌株Flavi.在生物控制策略中的潜在实用性.

关键词:

非洲毒素-B1 抗氧化剂是一种抗氧化剂.具有氧化作用的阿斯伯吉洛斯 (Aspergillus)有毒的有毒性阿斯伯吉洛斯 (Aspergillus)

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