转录因子VM1G_06867:在Valsa mali中增长,致病性,发育和维持细胞壁完整性的要求
Yufei Diao1, Jiyang Jin2, Xiong Xiong2
1Shandong Research Center for Forestry Harmful Biological Control Engineering and Technology, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China.
Journal of fungi (Basel, Switzerland)
|June 27, 2023
概括
指转录因子VM1G_06867对于瓦尔萨马利的生长,致病性和细胞壁完整性至关重要. 它部分挽救了VmSom1删除引起的缺陷,突出显示了它在果癌症中的作用.
科学领域:
- 植物病理学 植物病理学
- 分子菌学 分子菌学.
- 菌类遗传学 菌类遗传学
背景情况:
- 由Valsa mali引起的果瘤病对中国的果种植构成重大威胁.
- 转录因子VmSom1通过cAMP/PKA通路调节关键病原体功能,包括生长,发育和病原性.
- 转录组分析发现VM1G_06867,一个指转录因子,在VmSom1删除突变中差异表达.
研究的目的:
- 为了研究Valsa mali基因VM1G_06867.7.的功能.
- 阐明VM1G_06867与之前识别的转录因子VmSom1.1之间的关系.
- 了解VM1G_06867在真菌生长,致病性,无性发育和细胞壁完整性中的作用.
主要方法:
- 通过同源重组生成VM1G_06867的单删除突变物.
- 构建一个双删除突变 (ΔVmSom1/06867) 来研究基因相互作用.
- 在各种介质 (PDA,SDS,刚果红色,NaCl,Sorbitol) 上对野生类型,单和双删除突变的表型分析,以评估生长,结合和应激耐受性.
主要成果:
- 与野生类型相比,VM1G_06867单 deletion 突变体表现出显著降低的生长率和增加的 pycnidia 形成.
- VM1G_06867删除赋予了对SDS,刚果红色和光亮化剂的敏感性,表明细胞壁完整性缺陷.
- 双删除突变体与VmSom1单一突变体相比,在生长或结合方面没有显著差异,但未能产生结合体,并且在透应激条件下表现出增长 (刚果红色,NaCl,Sorbitol).
结论:
- VM1G_06867对于瓦尔萨马利的生长,致病性,无性发育和维持细胞壁完整性至关重要.
- VM1G_06867在克服透应激和细胞壁缺陷方面发挥着至关重要的作用.
- VM1G_06867可以部分恢复由VmSom1删除引起的致病性和应激耐受性缺陷,这表明这两种转录因子之间存在功能关系.
相关概念视频
Bacterial Cell Wall
72
The bacterial cell wall is an essential structural component that encases the plasma membrane, preserving cellular integrity, determining shape, and protecting against osmotic stress. This rigid yet flexible structure primarily comprises peptidoglycan, a polymer that forms a mesh-like matrix conferring mechanical strength and flexibility.Peptidoglycan Composition and StructurePeptidoglycan, the core of the bacterial cell wall, comprises alternating units of N-acetylglucosamine (NAG) and...
72
Gene Regulation During Sporulation
41
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
41
Archaeal Cell Wall
54
Archaeal cell walls are structurally and compositionally distinct from their bacterial counterparts, lacking the characteristic peptidoglycan layer found in most bacteria. Instead, archaeal cell walls exhibit remarkable diversity, utilizing materials such as pseudomurein, polysaccharides, and proteins to construct their protective outer layers. This structural flexibility is closely tied to archaea's ecological adaptability.S-Layers: The Common Archaeal Cell WallThe S-layer is the most...
54
Plant Cell Wall
56.9K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
56.9K
Stringent Response in E. coli
32
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
32
Transcription Attenuation in Prokaryotes
15.6K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.6K


