液体染色学-并联质谱法用于检测和定量口服液体样本中的梅洛西卡姆和5'-碳素梅洛西卡姆
Gabriela Moraes Oliveira1, Thiago José Dionísio2, Viviane Silva Siqueira-Sandrin2
1Hospital for Rehabilitation of Craniofacial Anomalies, University of São Paulo (HRAC/USP), Bauru 17012-900, SP, Brazil.
Metabolites
|June 27, 2023
概括
一种新的液体染色学-并联质谱法 (LC-MS/MS) 方法快速量化了梅洛西卡姆及其代谢物在口服液中的含量. 这种经过验证的方法使药理动力学/药理动力学研究成为可能,为体内药物的行为提供了洞察力.
科学领域:
- 分析化学 分析化学
- 药理动力学 药理动力学
- 生物医学科学 生物医学科学
背景情况:
- 梅洛西卡姆是一种广泛使用的非类固醇抗炎药物 (NSAID).
- 在生物矩阵中精确量化梅洛西卡姆及其代谢物对于治疗药物监测和药理动力学研究至关重要.
- 口服液为药物分析提供了非侵入性的血液替代品.
研究的目的:
- 开发和验证一种灵敏,选择性和快速的液体染色学-并联质谱法 (LC-MS/MS) 方法,用于在人类口腔液体中确定梅洛克萨姆及其主要代谢物5'-碳素梅洛克萨姆.
- 评估使用这种方法用于药理动力学/药理动力学 (PK/PD) 研究的可行性.
主要方法:
- 使用Shim-Pack XR-ODS列的LC-MS/MS方法的开发和验证.
- 从志愿者中采集了口服液样本,这些样本是在梅洛西卡姆在96小时内服用后采集的.
- 用Phoenix WinNonlin软件确定了药理动力学参数.
- 进行了前列腺素E2 (PGE2) 的量化,以探索PK/PD相关性.
主要成果:
- 开发的LC-MS/MS方法表现出高灵敏度,选择性和速度,总运行时间为5分钟.
- 验证参数包括线性,准确性,精度,稳定性和量化极限 (LLOQ:0.6103 ng/mL) 在可接受范围内.
- 在口服液中成功确定了梅洛西卡姆和5'-carboxymeloxicam度,从而可以进行药理动力学分析.
- 实现了PGE2的检测和量化,支持了PK/PD调查的潜力.
结论:
- 经过验证的LC-MS/MS方法适用于在口服液中快速可靠地定量梅洛西卡姆及其代谢物.
- 这种方法促进了梅洛西卡姆的非侵入性药理学和潜在的药理学/药理学研究.
- 该方法的稳定性和准确性支持其在临床和研究环境中的应用.
关键词:
5′-炭基梅洛西卡姆的使用.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量梅洛西卡姆是什么意思 梅洛西卡姆口服液体是指口服液体.更多相关视频
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