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鉴定了一种新型的脂酸生物合成途径,揭示了 prokaryotes 中的脂酸组合的复杂演变

Tomohisa Sebastian Tanabe¹, Martina Grosser¹, Lea Hahn¹

¹Institut für Mikrobiologie & Biotechnologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Germany.

|June 27, 2023

概括

研究人员发现了一种新的细菌路径,用于脂酸组装,涉及sLpl(AB) 联酶和LipS1/LipS2蛋白. 这一发现扩大了我们对酸盐生物发生进化的理解.

科学领域:

生物化学生物化学
进化生物学进化生物学
微生物学微生物学

背景情况:

脂酸是中央碳代谢和硫氧化过程中必不可少的重要生物分子.
在真核生物和一些原生动物中现有的酸盐组装途径是原生生物的起源.
酸盐生物发生路径的进化历史和多样性仍然不完全理解.

研究的目的:

为了识别和描述细菌中的新型脂酸盐组装途径.
阐明不同脂酸盐生物发生系统的进化起源和分布.
为了解脂酸代谢的演变提供一个全面的框架.

主要方法:

实验验证一种新的细菌脂酸盐组装途径.
广泛的同源性搜索和基因组上下文分析.
大规模的遗传学分析,绘制生命树上的路径.

主要成果:

发现了一种利用sLpl(AB) 脂酸:蛋白联酶和LipS1/LipS2蛋白的新细菌通路.
识别了比以前已知的更广泛的酸盐生物发生系统分布.
有证据表明,酸盐组装酶的模块化性质具有多种组合.
遗传学分析表明,古生物是新途径的关键组成部分的可能来源.

结论:

新的sLpl(AB) -LipS1/S2通路在细菌中广泛存在,扩大了已知的酸盐生物发生的多样性.
专用脂酸盐组装和清理机械的早期演变.
复杂的水平基因转移,基因获取,融合和损失塑造了这些途径在原核生物中的分布.
古代LplAB酶是细菌sLpl(AB的祖先,LipS1/S2也可能起源于古生物.

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