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乳腺癌中的PAK1拷贝数 - 与增殖和分子亚型的关联
Anette H Skjervold1, Marit Valla1,2, Borgny Ytterhus1
1Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, Trondheim, Norway.
PloS one
|June 27, 2023
概括
增加P21激活激酶1 (PAK1) 基因拷贝数量与乳腺癌的更高的扩散和分级有关,特别是在HER2阳性和光B亚型中,但不是预后. 这一增长也与CCND1副本编号的变化有关.
科学领域:
- 在瘤学瘤学.
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- P21激活激酶1 (PAK1) 在各种人体瘤中过度表达,包括乳腺癌 (BC).
- PAK1位于11号染色体 (11q13.5-q14.1) 上,在BC增殖中起着至关重要的作用.
- 两种PAK1和CCND1基因都位于染色体11q13.3上.
研究的目的:
- 在初级乳腺瘤和淋巴结转移中评估PAK1基因拷贝数 (CN).
- 调查PAK1 CN与扩散状态,分子亚型和预后之间的关联.
- 为了研究PAK1和CCND1.1的副本编号之间的关联.
主要方法:
- 在512BC组织微阵列上用于PAK1和CEP11的光 in situ杂交 (FISH).
- 通过计算20个瘤细胞核中的光信号,量化了PAK1和CEP11的副本数量.
- 统计分析包括皮尔森的千平方测试和预后生存分析.
主要成果:
- 在9.4%的瘤中观察到PAK1拷贝数增加 (平均CN≥4).
- 在HER2型和Luminal B (HER2-) 亚型中,PAK1 CN的增加最为普遍.
- PAK1 CN增加与高增殖率和组织学等级相关,但预后不确定的. 30%的PAK1 CN ≥6病例中,也有CCND1 CN ≥6.6的情况.
结论:
- 增加PAK1拷贝数量与乳腺癌的高增殖率和组织学等级有关.
- 这种关联在HER2型和Luminal B (HER2-) 亚型中最常见.
- PAK1拷贝数量的增加与CCND1拷贝数量的增加有关,但不是预后.
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