病原体蛋白质模块化使宿主酸酶的复杂模仿成为可能
Hui Li1, Jinlong Wang2, Tung Ariel Kuan3
1The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich NR4 7UH, UK.
Cell
|June 27, 2023
概括
病原体效应器使用保存的 (L) WY-LWY模块来劫持宿主蛋白酸酶2A (PP2A). 这种模块化允许多种效应器针对不同的宿主过程,揭示病原体的分子模拟策略.
科学领域:
- 植物病理学
- 分子生物学
- 结构生物学
背景情况:
- 病原体使用效应蛋白来操纵宿主细胞,但效应蛋白的功能多样性仍然不清楚.
- 植物病原体Phytophthora使用含有保存 (L) WY图案的效应器,它们表现出序列可变性.
研究的目的:
- 在Phytophthora效应器中研究 (L)WY图案的功能意义.
- 了解病原体的功能多样性是如何产生的.
- 阐明效应器与宿主蛋白相互作用的机制.
主要方法:
- 在多个Phytophthora效应器中识别特定的 (L)WY-LWY功能模块.
- 生物化学试验以证明蛋白质酸酶2A (PP2A) 核心酶的招募.
- 一个效应器-PP2A复合物的晶体结构的确定.
主要成果:
- 一个特定的 (L) WY-LWY组合作为一个功能模块,用于在植物宿主中有效地招募PP2A.
- 结构分析显示, (L) WY-LWY模块促进宿主PP2A核心酶的劫持以形成功能全酶.
- 具有共享N端PP2A相互作用模块的效应器表现出不同的C端LWY单元,调节不同的蛋白.
结论:
- 病原体通过分子模拟来适应像PP2A这样的基本宿主酸酶.
- 蛋白质模块化,以 (L) WY-LWY模块为例,推动了病原体效应器的功能多样化.
- 了解效应体与宿主之间的相互作用,可以了解病原体的毒性策略.
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