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肥胖BFMI小鼠脂肪组织的菌株和仓库特异性差异

Ayca Dogan¹, Ilke Sen², Mete Severcan³

¹Department of Physiology, Faculty of Medicine, Altinbas University, Istanbul, Turkey.

In vivo (Athens, Greece)

|June 27, 2023

概括

肥胖会改变脂肪组织的结构和功能. BFMI860小鼠系列显示显著的蛋白质变性,使其成为肥胖研究的理想选择.

科学领域:

分子生物学分子生物学
生物化学生化学
遗传学是一个遗传学.

背景情况:

肥胖与脂肪组织中的分子和结构变化有关.
了解这些变化对于代谢疾病研究至关重要.

研究的目的:

为了研究肥胖小鼠的 inguinal 和 gonadal 脂肪组织的分子和结构变化.
评估炎症性细胞因子和代谢因子分泌.
确定适合肥胖研究的小鼠模型.

主要方法:

使用了柏林脂肪老鼠杂交 (BFMI) 线和DBAJ对照老鼠.
使用qRT-PCR对关键细胞因子 (勒,IL-6,Tnf-α,Igf-1) 和Pparγ-2进行了mRNA表达分析.
分析脂肪组织中的蛋白质二次结构,使用ATR-FTIR光谱学与ANN和衍生向量正常化相结合.

主要成果:

肥胖的BFMI小鼠线表现出高水平的瘦素耐药性.
在BFMI线路中观察到测量因素 (不包括Igf-1) 的显著差异.
蛋白质二次结构分析显示随机卷轴含量增加,表明变性,特别是在BFMI860.0.

结论:

由于明显的分子变化,BFMI860系列是肥胖研究中最合适的模型.
肥胖对脂肪组织的影响取决于位置和直线.

关键词:

美国的FTIR是FTIR.肥胖问题肥胖问题发生性腺脂肪发生性腺脂肪部脂肪在部脂肪.勒普丁是一种素.蛋白质蛋白质是蛋白质蛋白质的组成部分.

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