结合性转移的链球菌的prophages 庇护抗生素耐药性和毒性基因
Jinhu Huang1, Xingyang Dai1, Zuowei Wu2
1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
The ISME journal
|June 27, 2023
概括
这项研究确定了链球菌中的SMphages,它们携带了许多抗微生物耐药性基因 (ARG),使其对十种药物类别产生耐药性. 这些菌体迅速传播,解释了链球菌感染中抗生素耐药性的普遍性.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 众所周知,食原体转移功能性质,但它们在传播抗微生物耐药性基因 (ARG) 中的作用仍在争论中.
- 链球菌种类是人类重要的病原体,了解ARG传播对于公共卫生至关重要.
研究的目的:
- 描述Streptococcus中普遍存在的prophages家族,称为SMphages.
- 研究SMphages所携带的抗菌素耐药性基因 (ARGs) 以及它们的传播机制.
主要方法:
- 鉴定SMphage基因组的特征,包括识别ARG和整合部位.
- 对SMphage集成和切除机制的分析.
- 使用细胞和动物模型验证SMphage编码的表面蛋白质毒性.
- 通过结合来研究SMphage转移,包括整合和结合元素的作用.
主要成果:
- 在SMphages中含有25种ARG,使其对10种抗微生物药物产生抗药性,包括菌素 (vanG) 和氧化丁 (optR).
- SMphages通过三个集成模块集成到四个染色体位点,并且可以诱导其消耗.
- 由SMphages编码的四种Alp相关表面蛋白的亚型在体外和体内表现出致命的效果.
- 高频结合促进了SMphage转移,得到了整合性和结合性元素的帮助.
结论:
- 在Streptococcus属中,SMphages是ARG广泛传播的重要因素.
- 已识别的ARG和毒性因素有助于链球菌的适应性和病原性.
- 了解SMphage生物学是打击这些重要细菌病原体抗菌素耐药性的关键.
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