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长非编码RNA的差异表达特征和功能分析在动脉疾病中的长非编码RNA

Guang-Yuan Song¹, Xu-Nan Guo², Jing Yao²

¹Interventional Center of Valvular Heart Disease, Beijing Anzhen Hospital Affiliated to Capital Medical University, Beijing, China. songgy_anzhen@vip.163.com.

BMC cardiovascular disorders

|June 27, 2023

概括

长非编码RNAs (lncRNAs) 参与了动脉膜疾病 (CAVD) 的病原发生. 这项研究确定了关键的lncRNA及其调节作用,为CAVD提供了潜在的诊断和治疗见解.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
心血管研究研究心血管研究

背景情况:

动脉疾病 (CAVD) 是一种进展性疾病,治疗选择有限.
长非编码RNAs (lncRNAs) 在CAVD病变发生中的作用在很大程度上仍未被探索.

研究的目的:

为了研究CAVD.中的lncRNAs的表达特征.
阐明CAVD.中已识别的lncRNA的潜在作用机制.

主要方法:

从基因表达总 (GEO) 数据库下载了基因表达特征.
利用差异基因表达分析,加权基因共同表达网络分析 (WGCNA) 和目标基因预测来识别关键的lncRNAs.
构建了一个具有竞争力的内源RNA (ceRNA) 网络,并进行了功能丰富分析.

主要成果:

在CAVD中确定了340个上调和460个下调的lncRNA.
确定了12个枢纽 lncRNAs,包括MIR4435-2HG,在CAVD病变发生过程中具有关键作用.
验证了这些枢纽 lncRNAs 在独立数据集中的作用,并通过 ceRNA 网络确认了 MIR4435-2HG 的关键功能.

结论:

lncRNAs在CAVD中发挥着重要作用,为了解疾病提供了新的视角.
已识别的 lncRNAs 可能作为 CAVD 诊断和治疗点的潜在生物标志物.
进一步的研究是有必要的,以充分理解这些lncRNAs在CAVD中的分子机制和治疗潜力.

关键词:

主动脉疾病主动脉疾病互联网监管 (Cis-regulation) 是一种对互联网的监管.具有竞争力的内源RNAs.长长的非编码RNAs.通过监管转变.在WGCNA中,WGCNA是WGCNA.

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