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转录组测序和miRNA-mRNA网络在胃腺癌M2巨细胞外体中的构建

Dun Pan^1,2,3, Zhipeng Li^1,2,3, Xin Lin^1,2,3

¹Department of Gastrointestinal Surgery, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, Fujian Province, China.

World journal of surgical oncology

|June 27, 2023

概括

研究人员在巨细胞外体中确定了一种关键的微RNA (miRNA) 和七种信使RNA (mRNA),这可能有助于诊断和治疗胃腺癌 (STAD). 这种巨细胞M2-miRNA-mRNA网络可以改善STAD患者的预后.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

胃腺癌 (STAD) 是最常见的胃癌类型.
巨细胞在瘤微环境中起着至关重要的作用.
识别巨相关的分子标记物对于STAD诊断和治疗至关重要.

研究的目的:

为了识别与STAD相关的巨细胞外体中的潜在分子标记物.
探索这些标记物在巨细胞M2极化中的作用.
为STAD预后构建一个miRNA-mRNA网络.

主要方法:

巨细胞外体的RNA测序以识别差异表达的miRNAs (DEmiRNAs).
对TCGA数据库对DEmiRNA和差异表达mRNA (DEmRNA) 的分析.
使用RT-PCR和在线数据集构建巨M2-miRNA-mRNA网络并进行验证.

主要成果:

确定了一种常见的miRNA,hsa-miR-133a-3p,与M2极化相关.
构建了一个由一个下调的miRNA (hsa-miR-133a-3p) 和七个上调的mRNA组成的核心网络.
通过RT-PCR和在线数据集验证的表达水平证实了网络的发现.

结论:

在巨细胞M2外体中发现的生物标志物可能会改善STAD患者的预后.
这项研究为STAD提供了潜在的诊断和治疗策略.
对这种miRNA-mRNA网络的进一步研究可以促进STAD治疗.

关键词:

外基因组是外基因组中的一个.巨细胞M2 的情况.有关RNA测序的RNA测序胃腺癌瘤是胃腺癌.它们是mRNARNA.这是一个小RNARNA.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...