相关实验视频
Updated: Jul 25, 2025
11:37
Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
Published on: July 8, 2010
12.0K
Fam20c通过酸化calpasatatin来调节calpain蛋白解系统,以维持细胞平衡
Xinpeng Liu1,2, Lili Jiang1,3, Wenxuan Zhang1
1Heilongjiang Provincial Key Laboratory of Hard Tissue Development and Regeneration, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Journal of translational medicine
|June 27, 2023
概括
具有序列相似性的20个成员C (FAM20C) 激酶家族对细胞功能至关重要. 缺少FAM20C会破坏骨质细胞平衡,影响细胞迁移和通过Calpastatin/Calpain系统传递Wnt信号.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生化学
背景情况:
- FAM20C 激酶酸化细胞外蛋白,调节细胞增殖,生物矿物化,迁移,粘附和酸盐平衡.
- 通过FAM20C调节下游分子的精确机制及其对细胞自主行为的影响以及基因型-表型相关性仍然不完全理解.
研究的目的:
- 研究FAM20C在骨质细胞功能中的作用背后的分子机制.
- 确定受FAM20C缺陷影响的关键调节器和信号通路.
主要方法:
- 使用测序 (ATAC-seq) 和RNA测序 (RNA-seq) 进行了对转化酶可访问的染色质的检测,这些检测是在Fam20c缺乏的骨质细胞上进行的.
- 蛋白质组学和光蛋白质组学被整合起来,以分析蛋白质的改变和翻译后的修改.
主要成果:
- 综合ATAC-seq和RNA-seq确定了TCF4和Wnt信号通路作为Fam20c缺乏细胞中的关键调节者.
- 蛋白质组学和光蛋白质组学揭示了Calpastatin/Calpain蛋白质解系统作为一种新的FAM20C标,可以调节细胞迁移和F-actin细胞骨架.
- 发现Calpastatin/Calpain系统对Wnt信号通路进行负面调节.
结论:
- 骨质母细胞中的FAM20C缺乏导致细胞平衡失衡.
- 多种信号通路,包括Wnt信号,在Fam20c淘汰骨质细胞中发生变化,突出显示了酶在细胞调节中的中心作用.
相关概念视频
01:16Calmodulin-dependent Signaling
5.2K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.2K
01:24Caspases
12.6K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
12.6K
02:54Protein Kinases and Phosphatases
13.2K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
13.2K
01:31Regulation of the Unfolded Protein Response
2.5K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.5K
02:58Regulated Protein Degradation
7.4K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.4K
01:27Feedback Regulation of Calcium Concentration
3.4K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.4K