在Neisseria meningitidis中对Beta-Lactams敏感性的最近演变
Ala-Eddine Deghmane1, Eva Hong1, Muhamed-Kheir Taha1
1Invasive Bacterial Infections Unit, Institut Pasteur, Université Paris Cité, 75724 Paris, France.
Antibiotics (Basel, Switzerland)
|June 28, 2023
概括
脑膜炎菌的抗生素耐药性正在上升,法国的青素耐药性显著增加. 由penA基因编码的PBP2蛋白的修改与这种对β-乳酸抗生素的敏感性降低有关.
科学领域:
- 微生物学 微生物学
- 遗传学 遗传学 是一个
- 药理学 药理学是指药理学的学科.
背景情况:
- β-乳糖抗生素对于治疗侵袭性脑膜炎球菌病至关重要.
- 人们越来越担心Neisseria meningitidis对青素G的耐药性增加.
- 还注意到对第三代头素 (3GC) 的敏感性降低以及ROB-1β-乳糖酶的存在.
研究的目的:
- 分析法国Neisseria meningitidis分离物中编码青素结合蛋白2 (PBP2) 的penA基因的修饰.
- 从2017年到2021年,追踪N. meningitidis中β-乳酸菌抗生素敏感性的趋势.
- 调查潜在的分子机制降低敏感性.
主要方法:
- 从2017年至2021年间培养的1255个N. meningitidis分离物中测序所有penA基因.
- 对青素和3GC降低敏感性的趋势分析.
- 鉴定和表征penA等位基因的.
- 野生类型和改变的PBP2氨基酸序列的分子建模.
主要成果:
- 对青素敏感性降低的比例从2017年的36%增加到2021年的58%.
- 降低对3GC的敏感性仍然很低,为2.4%.
- 鉴定了74种不同的penA等位基因;penA9是最常见的改变等位基因 (17%).
- A327等位基因与降低3GC敏感性有关.
- 在PBP2中氨基酸替代被证明可以阻碍β-乳酸结.
结论:
- A的修改是N. meningitidis中β-乳酸盐耐药性的主要机制.
- 特定的penA等位基因的流行率上升与青素耐药性的上升有关.
- 了解这些遗传变化对于指导抗生素治疗策略至关重要.
相关概念视频
Development of Antibiotic Resistance
46
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
46
Defense Against Bacterial Pathogens
1.5K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.5K
Antibiotic Selection
54.8K
Overview
54.8K
Transduction
49
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
49
Bacterial Cell Wall
72
The bacterial cell wall is an essential structural component that encases the plasma membrane, preserving cellular integrity, determining shape, and protecting against osmotic stress. This rigid yet flexible structure primarily comprises peptidoglycan, a polymer that forms a mesh-like matrix conferring mechanical strength and flexibility.Peptidoglycan Composition and StructurePeptidoglycan, the core of the bacterial cell wall, comprises alternating units of N-acetylglucosamine (NAG) and...
72
Mismatch Repair
40.4K
Overview
40.4K


