在家禽中转移抗菌素耐药性的必要条件
Aaron Oxendine1, Allison A Walsh1, Tamesha Young1
1School of Animal Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24060, USA.
Antibiotics (Basel, Switzerland)
|June 28, 2023
概括
禽类垃圾中含有显著的抗菌素耐药性 (AMR) 基因. 然而,该研究发现,在常见的实验室条件下,即使添加了细菌,对沙门氏菌等病原体的AMR传播潜力很低.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 基因转移 基因转移 基因转移
背景情况:
- 动物便是抗菌素耐药性 (AMR) 基因的储存库.
- 潜在的AMR基因溢出到人类通过病原体是令人担忧的.
- 禽 litter 微生物群在AMR传播中的作用需要研究.
研究的目的:
- 评估家禽垃圾微生物群传播AMR的能力.
- 量化家禽垃圾中的AMR和AMR基因负载.
- 为了研究AMR等离子体转移在家禽子中的微观世界.
主要方法:
- 使用抗生素敏感性测试评估的表型抗微生物耐药性 (AMR).
- 定量PCR (qPCR) 用于确定相对于16S rRNA的AMR基因负载 (aadA1, sul1) 的定量PCR.
- 在体外和微观实验中使用大肠杆菌和沙门氏菌来评估等离子体转移.
主要成果:
- 禽类垃圾对链杆菌素和四环素有很高的耐药性.
- 相对于细菌基因组,检测到显著的AMR基因负载 (aadA1, sul1).
- 在微观中没有检测到沙门氏菌的AMR,也没有观察到塑体转移到沙门氏菌.
结论:
- 禽类便中的微生物群拥有大量的AMR基因储备.
- 在测试条件下,从家禽垃圾传播到病原体的AMR传播潜力很低.
- 需要进一步的研究,以了解在不同的环境环境中AMR的动态.
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