光周期诱导DNA甲基化 发生在母羊的黑素受体1A基因中的变化
Xiaoyun He1, Wei Wang1, Wei Sun2,3
1Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Animals : an open access journal from MDPI
|June 28, 2023
概括
绵羊繁殖的季节性变化与MTNR1A基因表达有关. 光周期影响MTNR1ADNA甲基化,影响基因表达,并提供有关季节性繁殖模式的见解.
科学领域:
- 生殖生物学 生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 动物科学动物科学
背景情况:
- 黑色素受体1A (MTNR1A) 对于羊的雌性周期和季节性繁殖至关重要.
- 季节或光周期对MTNR1ADNA甲基化的影响仍未得到充分研究.
研究的目的:
- 研究光周期对羊MTNR1A基因表达和DNA甲基化的影响.
- 确定MTNR1A基因的核心促进子区域并分析其甲基化状态.
主要方法:
- 定量PCR (qPCR) 和西式斑点检测用于测量绵羊下丘脑中MTNR1A表达.
- 激光测序以确定MTNR1A核心促进体中的DNA甲基化水平.
- 在不同的光周期下使用了卵巢切除和雌激醇治疗 (OVX + E2) 的羊模型.
主要成果:
- 在短 (SP42) 和长 (LP42) 光周期治疗之间,MTNR1A基因表达有显著的差异.
- 核心MTNR1A促进区域被确定在转录起点 (TSS) 上游540 bp以内.
- 在不同光周期组中,在促进体区域的CpG位点观察到DNA甲基化水平的显著差异.
- 在MTNR1A基因表达和其DNA甲基化水平之间发现了强烈的负相关性.
结论:
- 光周期显著影响羊的MTNR1A基因表达和DNA甲基化.
- 促进MTNR1A基因的DNA甲基化是调节绵羊季节性繁殖的关键机制.
- 这些发现为进一步研究绵羊季节性繁殖的表观遗传调节提供了基础.
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