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猪中SINE插入碳酸无水素5B (CA5B) 基因与基因表达和表型变异的变化有关
Yao Zheng1, Cai Chen1,2, Mengli Wang1
1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Animals : an open access journal from MDPI
|June 28, 2023
概括
在猪CA5B基因中插入A SINE逆转移素会影响基因表达和脂肪沉积. 这种遗传变异与猪背部脂肪厚度增加有关,为脂肪组织发育提供了洞察力.
科学领域:
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
- 动物科学动物科学
背景情况:
- 转体子,特别是逆转体子,构成了哺乳动物基因组的很大一部分,包括猪基因组.
- 之前的研究发现,在猪中,CA5B基因的第一个内子内存在SINE逆转移素插入.
研究的目的:
- 在各种猪品种和野猪中调查CA5B基因中的SINE插入多态性.
- 阐明SINE插入对CA5B基因表达及其与脂肪组织特征相关性的功能影响.
主要方法:
- 聚合酶链反应 (PCR) 用于检测11种猪品种和野猪的SINE插入多态性.
- 进行了细胞实验,包括促进剂活性测定和增强剂验证,以评估SINE的调节功能.
- 进行了基因型和关联分析,以将SINE基因型与脂肪组织特征 (如背部脂肪厚度) 联系起来.
主要成果:
- 在11种研究的猪品种中,有9种观察到中等多态信息含量.
- 在RIP-CA5B部位的330bp SINE插入被发现可以增强CA5B基因的促进子区域的表达活性.
- 与SINE-/-个体相比,SINE插入 (SINE+/+) 个体在脂肪组织中表现出明显更高的CA5B表达和更大的背部脂肪厚度.
结论:
- 在CA5B基因中插入SINE作为增强剂,显著影响基因表达,并导致猪背部脂肪厚度增加.
- 这项研究揭示了一种涉及逆转移素插入的遗传机制,它影响了猪的脂肪组织发育和脂肪沉积.
- 在SINE插入中发现的ATTT重复为研究猪的遗传多样性和育种应用提供了潜在的标记.
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