相关实验视频

Updated: Jul 25, 2025

High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity

High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity

Published on: June 2, 2014

急性髓性白血病表达特定组的嗅觉受体

Gabriela D A Guardia¹, Rafaella G Naressi^2,3, Vanessa C Buzzato¹

¹Centro de Oncologia Molecular, Hospital Sírio-Libanês, São Paulo 01308-060, SP, Brazil.

|June 28, 2023

概括

研究人员在急性髓性白血病 (AML) 样本中确定了19个独特表达的嗅觉受体 (OR). 这一发现为AML患者提供了潜在的新诊断生物标志物和治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

急性髓性白血病 (AML) 的生存率很低,复发是主要的死亡原因.
目前的疗法有利于有限的患者亚组,突出需要更广泛的治疗点.
嗅觉受体 (ORs),通常在鼻子,越来越多地发现在非嗅觉组织,包括癌症.

研究的目的:

研究急性髓性白血病 (AML) 中嗅觉受体 (OR) 的表达模式.
确定特定的ORs,可以作为AML的诊断生物标志物或治疗点.
探索OR签名在AML患者的预后潜力.

主要方法:

在151个AML样本,来自15种其他癌症类型的6400个样本和11,200个健康组织样本中分析OR表达.
使用RT-PCR在独立的AML样本,健康的捐赠者和白血病细胞系上对已识别的OR进行实验验证.
生物信息分析用于识别AML中与ORs共同表达的癌症相关基因.

主要成果:

识别了19个OR,在AML中具有明显和主要的表达模式.
实验验证证证实了这些19个OR在AML中的表达.
发现一个OR签名,对AML患者有预后价值.
在AML中与这些ORs共同表达的癌症相关基因的识别.

结论:

嗅觉受体代表了用于AML诊断的新型生物标志物.
在这项研究中确定了特定的OR,它们有可能成为AML的治疗点.
或签名可以帮助预测患者的预后和指导治疗策略.

关键词:

急性骨髓性白血病 (AML) 是一种急性骨髓性白血病.癌症癌症癌症癌症癌症基因表达生物标志物生物标志物嗅觉受体是一种嗅觉受体.

更多相关视频

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

Published on: October 2, 2017

Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas

Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas

Published on: February 28, 2019

相关概念视频

Olfactory Receptors: Location and Structure

Olfactory Receptors: Location and Structure

The process of olfaction, also known as the sense of smell, is a sophisticated chemical response system. The specialized sensory neurons that facilitate this process, known as olfactory receptor neurons, are situated in an upper segment of the nasal cavity, known as the olfactory epithelium. Olfactory sensory neurons are bipolar, with their dendrites extending from the epithelium's apex into the mucus that lines the nasal cavity. Airborne molecules, when inhaled, traverse the olfactory...

Olfaction

Olfaction

The sense of smell is achieved through the activities of the olfactory system. It starts when an airborne odorant enters the nasal cavity and reaches olfactory epithelium (OE). The OE is protected by a thin layer of mucus, which also serves the purpose of dissolving more complex compounds into simpler chemical odorants. The size of the OE and the density of sensory neurons varies among species; in humans, the OE is only about 9-10 cm2.
The olfactory receptors are embedded in the cilia of the...

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...