开发一种自动在线流细胞计法,以量化细胞密度和指纹细菌群落的数量
Juan López-Gálvez1, Konstanze Schiessl2, Michael D Besmer2
1Department of Environmental Microbiology, Helmholtz-Centre for Environmental Research, Permoserstraße 15, D-04318 Leipzig, Germany.
Cells
|June 28, 2023
概括
这项研究引入了一种自动流细胞计方法,用于实时细菌社区分析. 该系统准确地测量细胞密度并区分细胞类型,使得人们能够快速了解微生物组动态和生物技术过程.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 分析化学 分析化学
背景情况:
- 精确的细胞密度测量对于微生物组研究和生物技术过程至关重要.
- 传统的离线细胞密度测定方法耗时,阻碍了快速干预.
- 现有的在线方法缺乏在细菌群体内区分各种细胞类型的能力.
研究的目的:
- 开发一种在线自动流细胞计程序,用于实时,高分辨率分析细菌群落.
- 为了同时在线计算细胞度和细菌细胞子集的分化.
- 为微生物组研究提供自动化数据分析和解释管道的工具.
主要方法:
- 将OC-300自动化装置与CytoFLEX流量细胞计相连接.
- 细菌样品的自动取样,稀释,固定和4',6-二胺-2-二英 (DAPI) 染色.
- 利用流式细胞计量来高分辨率测量细菌细胞密度和社区组成.
主要成果:
- 开发的方法可复制地测量细胞密度和独特的社区模式.
- 该系统生成适合自动化分析的高分辨率数据.
- 将数据自动分成单元子集的自动分区有助于进行相关性分析.
结论:
- 在线自动流细胞计程序为实时细菌社区分析提供了强大的解决方案.
- 这种方法可以识别导致社区骚乱或状态变化的因素.
- 它增强了对生物体相互作用和微生物群中的表现的理解.
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