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乌比奎丁-蛋白酶体系统在野猪精子容量化过程中参与精子表面亚蛋白质体重塑
Michal Zigo1, Karl Kerns1,2, Peter Sutovsky2,3
1Division of Animal Science, University of Missouri, Columbia, MO 65211, USA.
Biomolecules
|June 28, 2023
概括
在电容化过程中,全方位蛋白酶体系统 (UPS) 积极重塑精子表面蛋白质体. 这一过程影响精子代谢和结合,为调节生育能力提供了新的目标.
科学领域:
- 生殖生物学 生殖生物学
- 蛋白质组学是指蛋白质组学.
- 细胞生物学 细胞生物学
背景情况:
- 精子电容对于受精至关重要,涉及显著的生化和生物变化.
- 精子表面蛋白质在电容化过程中经历动态重塑.
- 在这种重塑过程中,无素-蛋白酶体系统 (UPS) 的作用尚未完全被理解.
研究的目的:
- 为了调查UPS参与修改精子表面子蛋白在野猪精子电容期间的参与.
- 识别精子表面的特定蛋白质,这些蛋白质在电容时由UPS调节.
主要方法:
- 从无能力和体外有能力 (IVC) 的猪精子中选择性地分离精子表面亚蛋白.
- 在IVC期间使用MG132进行保护体抑制.
- 高分辨率的定量液体染色学-质谱学 (LC-MS) 分析.
- 使用免疫细胞化学,流细胞测量和西式涂抹来验证蛋白质变化.
主要成果:
- 在精子表面识别了1680个HUGO注释蛋白.
- 在IVC精子表面发现了91种显著少量的蛋白质和141种显著更多的蛋白质.
- 在蛋白质体抑制时观察到14种蛋白质的显著丰度变化,包括NIF3L1,CSE1L和PFDN4.
- 通过UPS调节精子代谢和通过表面蛋白调节结合的经UPS调节.
结论:
- 在野猪精子电容化期间,UPS在精子表面蛋白质的广泛重塑中发挥着积极的作用.
- UPS调节的蛋白质参与了代谢,结合和受精等关键功能.
- 这项研究提供了对调节精子受精能力的潜在药理目标的见解.
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