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OTUD7B通过与LEF1的相互作用激活了Wnt信号通路
Yuri Lee1, Hai-Long Piao2, Jongchan Kim1
1Department of Life Sciences, Sogang University, Seoul 04107, Republic of Korea.
Biomolecules
|June 28, 2023
概括
研究人员发现,OTUD7B通过与LEF1.1相互作用来激活Wnt信号通路. 这种互动促进了LEF1的发展.
科学领域:
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
- 在瘤学瘤学.
背景情况:
- Wnt信号通路对于细胞功能如增殖,分化和亡至关重要.
- 异常的Wnt信号与各种疾病有关,特别是癌症,β-catenin和LEF1是关键的媒介.
- 脱基酶 (DUBs) 是一种可以去除基的酶,在蛋白质调节中起作用.
研究的目的:
- 通过LEF1蛋白识别调节Wnt信号通路的二维基提纳酶 (DUB).
- 调查OTUD7B影响Wnt信号的特定机制.
主要方法:
- 对人类DUB库进行选,以识别与LEF1.1交互的DUB.
- 同免疫沉和域分析以确认OTUD7B-LEF1相互作用.
- 免疫光检测评估OTUD7B对LEF1核定位的影响.
- 定量PCR (qPCR) 阵列分析以评估Wnt目标基因表达.
主要成果:
- OTUD7B被确定为与Wnt信号交互并激活的DUB.
- OTUD7B和LEF1之间的相互作用分别涉及UBA和HMG领域.
- OTUD7B增强了LEF1的核定位,增加了它与核中的β-catenin的相互作用.
- 过度表达OTUD7B可以调节75%的Wnt目标基因.
结论:
- OTUD7B通过促进LEF1核转位和与β-catenin的相互作用来积极调节Wnt信号.
- OTUD7B在激活Wnt信号传递中的作用表明,它可能是Wnt失调癌症的治疗点.
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