血清/氨酸蛋白酸酶1和2A在肺内皮屏障调节中的作用
Rahul S Patil1, Anita Kovacs-Kasa1, Boris A Gorshkov1
1Vascular Biology Center, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA.
Biomedicines
|June 28, 2023
概括
蛋白酸酶 (PPases) 对于维持肺部内皮细胞屏障完整性至关重要. 本综述强调了它们在调节细胞骨和透性方面的作用,特别是蛋白质酸酶1 (PP1) 和2A (PP2A).
科学领域:
- 内皮细胞生物学 内皮细胞生物学
- 血管屏障功能的功能
- 肺部医学 肺部医学
背景情况:
- 血管屏障功能障碍,标志着内皮细胞 (EC) 透性和炎症的增加,是急性肺损伤 (ALI),急性呼吸困扰综合征 (ARDS),败血症和COVID-19的核心原因.
- 对于肺功能至关重要的EC屏障完整性,依赖于细胞骨元素,细胞矩阵粘附和细胞间连接.
- 细胞骨重塑涉及Ser/Thr酸化/脱酸化,但Ser/Thr蛋白酸酶 (PPases) 在EC屏障调节中的作用尚未得到充分探索.
研究的目的:
- 审查Ser/Thr蛋白酸酶 (PPases) 在调节肺EC细胞骨和透性的作用.
- 要强调蛋白质酸酶1 (PP1) 和蛋白质酸酶2A (PP2A) 在这个过程中的特定作用.
- 整合PPase功能与细胞骨动力学和影响EC透性和炎症的信号级联.
主要方法:
- 文献综述侧重于Ser/Thr蛋白酸酶 (PPases) 及其对内皮细胞 (EC) 屏障功能的影响.
- 对细胞骨调节,酸化/脱酸化事件以及EC透性的现有研究进行分析.
- 在肺 EC 背景下整合与蛋白质酸酶1 (PP1) 和蛋白质酸酶2A (PP2A) 相关的发现.
主要成果:
- 建议Ser/Thr PPases抵消EC收缩,从而保持EC屏障的完整性.
- 虽然EC细胞骨酸化得到了充分的研究,但对PPases的脱酸化作用的理解较少.
- PP1和PP2A被确定为主要的哺乳动物Ser/Through PPases,对EC屏障功能有重大影响.
结论:
- 血/透酶,特别是PP1和PP2A,在维持肺内皮细胞屏障完整性方面发挥着至关重要的,但被低估的作用.
- 需要对PPase子单元,目标和监管机制进行进一步的研究,以充分了解它们在EC障碍监管中的功能.
- 了解PPase活性对于开发针对肺部疾病血管屏障功能障碍的治疗策略至关重要.
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