转录组网络揭示了日本鱼 (Paralichthys olivaceus) 组织特异性的冷冲击反应
Jiayi He1, Qing Zhu1,2, Ping Han1
1Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, 5 Yushan Road, Qingdao 266003, China.
Biology
|June 28, 2023
概括
低温对水生动物产生影响. 这项研究揭示了日本 (Paralichthys olivaceus) 对寒冷压力表现出组织特异的转录组反应,影响细胞功能和潜在的水产养殖生产力.
科学领域:
- 水生动物生理学 水生动物生理学
- 环境压力反应环境压力反应
- 分子生物学分子生物学
背景情况:
- 低温显著影响水生动物的分布,生存,生长和生理.
- 日本 (Paralichthys olivaceus) 是东亚的一个重要的水产养殖物种,易受温度波动的影响.
研究的目的:
- 为了研究日本鱼对急性寒冷压力 (10°C) 的协调转录基因反应.
- 在关键器官 (,心脏,肝脏,脏) 中,识别导致寒冷耐受和应激反应的组织特异性分子机制.
主要方法:
- 对日本鱼经过急性寒冷压力的,心脏,肝脏和脏进行了转录组分析.
- 权重基因共同表达网络分析 (WGCNA) 用于识别冷反应模块 (CRM).
- 组织学检查,以评估冷震后的组织损伤.
主要成果:
- 组织学分析表明不同程度的冷冲击损伤,主要是在和肝脏.
- 确定了10个组织特异性CRM,详细描述了细胞对寒冷压力的反应.
- 上调的CRM被丰富了细胞外基质和细胞骨等功能,而下调的CRM涉及细胞循环和DNA复合体,表明广泛的细胞干扰.
结论:
- 寒冷压力诱导了日本鱼的组织特异性转录基因反应,对所有研究的组织中的细胞功能产生了重大影响.
- 在细胞循环和DNA复合体等细胞过程中观察到的干扰突出了在寒冷条件下水产养殖生产力的潜在挑战.
- 这些发现提供了对日本鱼低温应激反应的分子基础的见解,这对于保护和养殖策略至关重要.
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