鼠标CircGHR调节肝细胞和肌细胞的增殖,分化和亡
Weilu Zhang1, Shudai Lin1, Zhenhai Jiao1
1College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
Genes
|June 28, 2023
概括
鼠标circGHR是一个来自生长激素受体基因的圆形RNA,显示了蛋白质编码的潜力. 这种循环RNA影响细胞增殖,分化和细胞亡,影响动物的生长.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 细胞生物学 细胞生物学
背景情况:
- 生长激素 (GH) -GHR-IGF1通路对于动物的生长和发育至关重要.
- 生长激素受体 (GHR) 的完整性会影响这种信号通路.
- 之前的一项研究发现了来自小鼠GHR基因的圆形转录,称为circGHR.
研究的目的:
- 为了研究小鼠circGHR的蛋白质编码潜力.
- 分析circGHR对细胞行为的功能影响.
- 了解circGHR在与生长相关的细胞过程中的作用.
主要方法:
- 生物信息预测circGHR的开放阅读框架.
- 为circGHR构建一个标记Flag的蛋白质载体.
- 西方斑点分析以验证蛋白质表达.
- 使用NCTC469和C2C12细胞系进行细胞增殖,分化和细胞亡测定.
主要成果:
- 预测CircGHR有一个开放的读取框架,表明编码潜力.
- 西方斑点证实了circGHR.的Flag标记蛋白的初步表达.
- 循环GHR抑制了NCTC469细胞的增殖,并显示出抑制亡的趋势.
- 循环GHR倾向于抑制增殖,并促进C2C12细胞的分化.
结论:
- 鼠标circGHR具有编码蛋白质的潜力.
- 环GHR影响关键的细胞过程,包括增殖,分化和亡.
- 这些发现表明,circGHR在影响动物发育的细胞功能中起着调节作用.
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