通过使用C-CRISPR有效和特定地产生MSTN编辑的胡羊
Rihong Guo1,2, Huili Wang1,2, Chunhua Meng1,2
1Jiangsu Provincial Engineering Research Center for Precision Animal Breeding, Nanjing 210014, China.
Genes
|June 28, 2023
概括
研究人员使用C-CRISPR系统,通过非激活MSTN基因,制造出具有双肌 (DM) 表型的转基因胡羊. 这种高效的方法有望改善畜牧养殖.
科学领域:
- 动物遗传学动物遗传学
- 基因编辑技术的技术
- 提高畜牧业的改善
背景情况:
- 胡羊是中国土著品种,具有高生育能力,但需要增强生长和尸体特征.
- 肌平素 (MSTN) 是肌肉发育的关键负调节剂;其无活化导致肌肉性.
- 通过使用多个导向RNA,C-CRISPR系统可以实现高效的,单步基因淘汰.
研究的目的:
- 使用C-CRISPR系统生成MSTN淘汰赛 (KO) 胡羊.
- 为了研究由此产生的双肌 (DM) 表型,并评估基因编辑的效率和特异性.
- 评估C-CRISPR在畜牧业动物繁殖中的潜力.
主要方法:
- Cas9 mRNA和四个单导向RNA针对绵羊MSTN外因子3被微注射到胡羊胚胎中.
- 胚胎被转移到受体母羊中,结果的羔羊被分析为MSTN基因编辑.
- 对编辑的羊进行了表型特征和分子分析 (AKT/ERK信号).
主要成果:
- 在10只出生的羊羔中,有9只出现了各种突变的完整的MSTN KO,这表明了高的编辑效率.
- 没有检测到非目标突变,这表明C-CRISPR系统的高特异性.
- MSTN-KO羊表现出明显的双肌表型,体重增加,以及改变的肌肉信号通路.
结论:
- 在C-CRISPR系统的有效性和特异性产生MSTN完整的KO胡羊与双肌的表型.
- 这项研究证实C-CRISPR是农场动物基因改造的强大工具.
- 开发的MSTN-KO胡羊模型为推进畜牧养殖计划提供了潜力.
相关概念视频
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