代谢组,植物荷尔蒙和转录组分析揭示了桃子肉中安托西亚宁的空间积累模式的机制
Ping Sun1, Chengkun Yang2,3, Wencan Zhu2,3
1Jinhua Academy of Agricultural Sciences (Zhejiang Institute of Agricultural Machinery), Jinhua 321000, China.
Foods (Basel, Switzerland)
|June 28, 2023
概括
桃肉颜色的变化与由特定的基因和植物激素调节的抗生素积累有关. 这项研究确定了控制桃子水果中这些色素的空间模式的关键分子参与者.
科学领域:
- 植物生物化学 植物生物化学
- 发展水果发展水果发展
- 分子遗传学 分子遗传学
背景情况:
- 安西是水果中重要的二次代谢物,影响着颜色和健康益处.
- 桃子 (Prunus persica) 肉体表现出氨酸积累的空间模式,但潜在的机制尚不清楚.
研究的目的:
- 阐明调节桃子皮中安东素的空间积累的分子机制.
- 为了确定关键的基因和植物激素参与红色和黄色桃子肉之间的差异色彩.
主要方法:
- 在桃子cv的红肉 (RF) 和黄色肉 (YF) 之间对黄素代谢物,植物激素和转录组数据的比较分析. "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "金秀". "
- RNA测序 (RNA-seq) 用于识别差异表达的基因,包括那些参与氨酸生物合成和调节的基因.
- 关键植物激素的量化,如奥克辛,细胞激素,ABA,SA和ACC.
主要成果:
- 丁-3-O-葡萄糖酸的积累驱动了桃子中骨中红色的变色.
- 在RF中,对氨酸生物合成基因 (F3H,F3'H,DFR,ANS),运输基因 (GST) 和调节基因 (MYB10.1,bHLH3) 的升级调节.
- 观察到植物激素 (RF中的auxin,cytokinin,ACC,SA;YF中的ABA) 和改变的auxin/cytokinin信号通路的差异积累.
结论:
- 确定了控制桃子中安托素生物合成的候选调节基因 (ERF,WRKY,NAC).
- 植物激素,特别是辅素和细胞因素信号传递,在桃子皮肤颜色的空间调节中发挥着重要作用.
- 提供了对复杂的遗传和荷尔蒙网络的新见解,这些网络控制了桃子中安东素的模式.
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