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在番茄成熟期间对下游监管网络的CHIP-Seq分析

Yanan He¹, Qiong Wu¹, Chunxiao Cui¹

¹Engineering Center of Ministry of Education, School of Food and Strategic Reserves, Henan University of Technology, Zhengzhou 450001, China.

Foods (Basel, Switzerland)

|June 28, 2023

概括

转录因子 SlAREB1 通过控制参与关键代谢途径的下游基因来调节番茄果实成熟. 这项研究确定了972个SLAREB1点,进步了我们对果实发育中的酸信号传导的理解.

科学领域:

植物分子生物学植物分子生物学
果实成熟生理学果实成熟生理学
亚酸信号传递亚酸信号传递

背景情况:

SlAREB1,一个酸 (ABA) 反应元素结合因子,与ABA调节的基因表达和番茄果实成熟有关.
规范这些过程的SlareB1的具体下游目标在很大程度上仍未被描述.

研究的目的:

识别和描述西红中SlarEB1的全基因组下游点.
为了阐明SlarEB1在番茄果实成熟中的调节网络.

主要方法:

染色体免疫沉测序 (ChIP-seq) 用于在番茄基因组中识别 SlAREB1 的结合点.
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析是在确定的目标基因上进行的.

主要成果:

在成熟的绿色阶段,slAREB1的表达达到顶峰,在成熟过程中下降.
ChIP-seq确定了SlarEB1的972个下游基因标,主要是在基因间和促进器区域.
目标基因在生物功能,氧化酸化,光合作用,植物激素合成,细胞壁修饰,色素生物合成和抗氧化途径方面得到显著丰富.

结论:

SlAREB1通过广泛的下游目标网络在调节番茄果实成熟方面发挥着重要作用.
确定的目标为了解ABA在水果发育和成熟方面的监管机制提供了基础.

关键词:

这就是ChIP-Seq.转录因子 SlAREB1 的转录因子.阿布西斯酸是什么?阿布西斯酸是什么果实成熟成熟的时间在这里,我们可以看到茄,番茄,番茄.

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