由于通过的减弱光,双固化树脂水泥的不完全聚合会诱导炎症反应
Takeru Kondo1,2, Hiroaki Kakinuma2, Kanna Fujimura1
1Division of Molecular & Regenerative Prosthodontics, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan.
International journal of molecular sciences
|June 28, 2023
概括
双固化树脂水泥的不完整的聚合,通过回复可以触发炎症反应. 这通过增加活性氧物种和细胞中激活的炎症途径发生.
科学领域:
- 生物材料科学 生物材料科学
- 牙科材料 牙科材料
- 免疫学 免疫学 免疫学
背景情况:
- 的修复在牙科中被广泛使用.
- 的阻光特性可以阻碍双固化树脂水泥的完全聚合.
- 不完整的聚合可能会导致未反应的单体的释放以及随后的不良生物效应.
研究的目的:
- 为了研究体外对双固化树脂水泥的炎症反应,通过的光衰减引起的不完全聚合.
- 评估石厚度对树脂水泥聚合和单体化的影响.
- 评估细胞机制,包括反应性氧物种 (ROS) 生成和基因激活蛋白 (MAP) 激酶激活,参与炎症反应.
主要方法:
- 双固化树脂水泥通过不同厚度 (1.0,1.5,2.0毫米) 的圆盘进行光固化.
- 测量了树脂水泥的光透度和转换度 (DC).
- 树脂单体 (基乙基甲酸盐,三甲醇二甲酸盐) 的化量化.
- 在人类牙纤维细胞 (hGF) 和单细胞细胞中分析了促炎性细胞因子 (IL-1β,IL-6,TNFα) 的基因表达.
- 评估了细胞内ROS水平和MAP激酶激活.
主要成果:
- 增加的厚度显著降低了树脂水泥的光透率和直流.
- 较高的单体化被观察到与1.5毫米和2.0毫米的合金厚度与没有合金.
- 在hGFs中IL-1β和IL-6的升级基因表达,在单细胞细胞中TNFα,被发现有更厚的.
- 不完整的聚合导致细胞内ROS水平升高,并在两种细胞类型中激活MAP激酶.
结论:
- 由于的光衰减,双固化树脂水泥的不完全聚合会导致牙纤维细胞和单细胞细胞的炎症反应.
- 这种炎症过程是由细胞内ROS生成和随后的MAP激酶路径激活的介导.
- 临床含义表明,要仔细考虑石厚度和水泥固化方案,以尽量减少不良的炎症反应.
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