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相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Types of RNA01:20

Types of RNA

5.9K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
5.9K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

13.3K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.3K
Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

1.4K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
1.4K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

951
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
951
RNA Interference01:23

RNA Interference

26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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相关实验视频

Updated: Jul 25, 2025

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
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Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

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非编码RNA和基生成

Wenxiu Ru1,2, Sihuan Zhang2, Jianyong Liu3

  • 1College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China.

International journal of molecular sciences
|June 28, 2023
PubMed
概括

这项研究探讨了脂肪生成,即脂肪细胞发育的过程,并强调了像lncRNA,miRNA和circRNA这样的非编码RNA (ncRNA) 在调节潜在肥胖治疗的复杂网络中的关键作用.

关键词:
脂质生成 (adipogenesis) 是一种环RNA 环RNA 是一个环RNA.在ncRNA中,我们可以看到这是一个小RNARNA.

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An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
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Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
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An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
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Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
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科学领域:

  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 细胞生物学 细胞生物学

背景情况:

  • 脂肪生成是一个复杂的过程,涉及许多转录因子和信号通路.
  • 人们越来越认识到表观遗传机制在调节脂肪细胞发育中的作用.
  • 非编码RNAs (ncRNAs),包括长非编码RNAs (lncRNAs),microRNAs (miRNAs) 和循环RNAs (circRNAs),是生物过程中的关键调节者.

研究的目的:

  • 概述脂肪生成的基本过程.
  • 讨论ncRNAs在脂肪细胞发育中的最新角色和调控机制.
  • 探索ncRNAs作为治疗肥胖和相关代谢疾病的治疗点的潜力.

主要方法:

  • 关于代和ncRNAs的最近研究的文献综述.
  • 对ncRNAs调节基因表达的分子机制的分析.
  • 综合目前对ncRNA参与脂肪细胞分化的理解.

主要成果:

  • ncRNAs通过与DNA,RNA和蛋白质的相互作用在多个层面上调节基因表达.
  • 特定的ncRNAs (lncRNA,miRNA,circRNA) 在控制脂肪生成中起着重要的作用.
  • 对ncRNAs的失调与肥胖和相关疾病的发展有关.

结论:

  • 了解ncRNA介导的脂肪生成调节提供了对代谢性疾病发病的新见解.
  • ncRNAs代表了开发针对肥胖的治疗策略的有希望的目标.
  • 对ncRNA功能的进一步研究可以推进代谢性疾病治疗领域.