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一种瘤治疗疗法VAX014可以减少腺瘤,并在CRC小鼠模型中修改结肠微环境
Shea F Grenier1, Mohammad W Khan1, Katherine A Reil2
1Department of Biology, Molecular Biology Institute, San Diego State University, San Diego, CA 92182, USA.
International journal of molecular sciences
|June 28, 2023
概括
VAX014,一种型细菌疗法,减少了结直肠癌 (CRC) 腺瘤和瘤. 这种新的治疗方法显示出在CRC中具有预防性和新辅助性治疗的潜力,利用球性和免疫治疗效应.
科学领域:
- 在瘤学瘤学.
- 免疫治疗是一种免疫疗法.
- 微生物学 微生物学
背景情况:
- 结肠直肠癌 (CRC) 是癌症死亡的主要原因,需要新的治疗方法.
- VAX014是一种瘤性细菌迷你细胞疗法,具有已证明的抗瘤免疫反应.
- 在CRC中VAX014的疗效尚未完全探索.
研究的目的:
- 在结直肠癌的临床前模型中评估VAX014的疗效.
- 评估VAX014作为CRC的预防和新辅助疗法.
- 研究VAX014的作用机制,包括瘤溶解和免疫调节.
主要方法:
- 在CRC细胞系上对VAX014进行体外试验,以进行瘤溶解.
- 在Fabp-CreXApcfl468小鼠模型中的体内评估,用于预防和新辅助治疗.
- 对瘤大小,数量,基因表达 (炎症,免疫标志物) 和增殖的分析 (Ki67).
主要成果:
- 在CRC细胞系中诱导VAX014瘤溶解.
- 预防性VAX014减少了腺瘤的大小和数量,而没有改变关键基因表达.
- 新辅助剂VAX014降低了瘤数量,调高了抗瘤TH1标志物,增加了Akkermansia muciniphila,并降低了Ki67的扩散.
结论:
- 在CRC模型中,VAX014在CRC模型中表现出瘤治疗和免疫治疗效应.
- 该疗法在预防腺瘤方面有望用于预防性使用.
- 新辅助剂VAX014在减少瘤和调节免疫微环境方面有效,支持其在CRC治疗中的潜力.
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