生物性金属氧化物纳米颗粒的抗菌活性及其对临床病原体的协同作用
Dali Vilma Francis1, Manju Nidagodu Jayakumar1,2, Hafiz Ahmad3,4
1Department of Biotechnology, Birla Institute of Technology and Science, Pilani, Dubai Campus, Dubai International Academic City, Dubai P.O. Box 345055, United Arab Emirates.
International journal of molecular sciences
|June 28, 2023
概括
新型生物性金属纳米粒子 (CuO,ZnO,WO3) 对抗药物耐药性病原体表现出显著的抗菌活性. 组合增强了疗效,光激活的WO3纳米颗粒证明特别有希望.
科学领域:
- 纳米技术 纳米技术
- 微生物学 微生物学
- 材料科学 材料科学 材料科学
背景情况:
- 抗生素耐药性是一个日益增长的全球健康威胁,需要开发新的抗菌剂.
- 生物性金属氧化物纳米粒子 (CuO,ZnO,WO3) 正在作为传统抗生素的潜在替代品进行研究.
研究的目的:
- 评估生物基CuO,ZnO和WO3纳米颗粒对临床分离物,包括耐药菌株的抗菌疗效.
- 探索这些纳米颗粒的协同效应和光催化抗菌活性,无论单独还是组合.
主要方法:
- 临床分离物 (大肠杆菌,黄金杆菌,MRSA,C.albicans) 在黑暗和光明条件下用单个和组合的金属纳米颗粒处理.
- 通过量化可活细胞来评估抗菌活性.
- 通过测量脂质过氧化 (MDA产生) 和细胞完整性 (活/死染色,流细胞计,光显微镜) 来研究作用机制.
主要成果:
- 生物基CuO和ZnO纳米颗粒在黑暗中表现出显著的抗菌作用,不受光激活的影响.
- 光激活的WO3纳米粒子在所有测试生物体中实现了75%的可活细胞减少.
- 与CuO,ZnO和WO3纳米颗粒的组合表现出协同效应,与单个纳米颗粒相比,抗菌性质增加了90%以上.
结论:
- 生物性金属氧化物纳米粒子,特别是光激活下的WO3和CuO,ZnO和WO3的组合,对抗多药耐药病原体表现出强大的抗菌活性.
- 这些纳米颗粒为对抗耐药细菌和真菌引起的感染提供了有希望的策略.
- 该研究阐明了涉及活性氧物种生成和细胞膜损伤的抗微生物机制.
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