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联合QTL映射和转录基因组测序分析揭示了常见小麦麦中与种子破碎相关的候选基因

Chuyi Chen¹, Yuke Zhang^1,2, Yang Liu¹

¹College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.

International journal of molecular sciences

|June 28, 2023

概括

在普通麦中,种子破碎是一个主要问题. 这项研究确定了21个与脚强度相关的候选基因,为通过分子育种开发耐碎麦品种提供了洞察力.

科学领域:

植物遗传学植物遗传学
农业科学农业科学
分子生物学分子生物学

背景情况:

常见的麦 (Fagopyrum esculentum) 是一种重要的谷物作物,但种子破碎显著限制了其产量.
了解种子破碎的遗传基础对于提高作物弹性和生产率至关重要.

研究的目的:

为了研究普通麦中种子破碎的遗传结构和调节.
确定与脚强度和破碎阻力相关的定量特征位点 (QTL) 和候选基因.

主要方法:

使用来自对比父母 (Gr和UD) 的 F2 种群构建遗传链接地图.
RNA测序 (RNA-seq) 和非向气体染色体质谱 (GC-MS) 用于基因表达和代谢物分析.
权重基因共同表达网络分析 (WGCNA) 和联合分析,以整合多omics数据.

主要成果:

检测到7个QTL用于脚强度,并在家长脚中确定了214个差异表达基因 (DEG).
代谢分析揭示了138种不同的代谢物,其中11种DEG与这些代谢物显著相关.
在QTL区域内共确定了43个基因,其中6个在脚中表现出高表达,从而选择了21个候选基因.

结论:

这项研究阐明了普通麦种子破碎的基因因素.
已识别的候选基因为标记器辅助的选择和耐碎品种的繁殖提供了有价值的目标.
这些发现有助于更深入地了解麦遗传学和分子育种策略.

关键词:

QTLs 是一个 QTL.在WGCNA中,WGCNA是WGCNA.常见的麦常见的麦这种种子破碎的种子.转录组和代谢组的测序.

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