感染SARS-CoV-2的患者IgG的催化酶活性
Anna S Tolmacheva1, Margarita K Onvumere1, Sergey E Sedykh1
1Institute of Chemical Biology and Fundamental Medicine, SB of the RAS, 630090 Novosibirsk, Russia.
International journal of molecular sciences
|June 28, 2023
概括
COVID-19幸存者显示出显著更高的血清IgG催化酶活性,这表明抗体可能有助于对抗有害的活性氧物种. 这一发现为COVID-19后的健康提供了新的见解.
科学领域:
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
- 病理生理学 病理生理学
背景情况:
- 冠状病毒疾病 (COVID-19) 带来了长期的健康挑战,包括多种表现的后COVID综合征.
- SARS-CoV-2 感染可以通过反应性氧物种 (ROS) 的过度生产诱导氧化应激,影响氧气运输和促进血栓形成.
- 抗体,包括免疫球蛋白G (IgGs),可以具有酶活性,如抗氧化功能,有助于细胞防御机制.
研究的目的:
- 为了研究和比较不同队伍的血清IgG触酶活性:COVID-19幸存者,斯普特尼克V接种疫苗的个人,接种了COVID-19幸存者和健康的捐赠者.
- 确定COVID-19感染是否影响血清IgG的抗氧化能力,特别是它们中和过氧化的能力.
主要方法:
- 从四个不同的群体收集血清样本:康复的COVID-19患者,用Sputnik V接种疫苗的健康志愿者,在COVID-19康复后接种疫苗的个人和健康的捐赠者.
- 血清IgG的相对催化酶活性被测量并使用生物化学测定对这些组进行比较.
主要成果:
- 从COVID-19中康复的患者的血清IgG表现出最高的催化酶活性.
- 这种活动在COVID-19幸存者中比健康捐赠者 (1.9倍),Sputnik V接种疫苗的个人 (1.4倍) 和接种了COVID-19幸存者 (2.1倍) 高出统计学上显著.
结论:
- 似乎COVID-19感染刺激了血清IgG的产生,并增强了catalase活性.
- 这些发现表明,抗体可能通过降解有害的过氧化在COVID-19感染后的感染来减轻氧化压力.
相关概念视频
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
01:29Coronavirus
Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...