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基于微RNA生物标志物的流产或死产预测模型的第一季度查

Ilona Hromadnikova¹, Katerina Kotlabova¹, Ladislav Krofta²

¹Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, 14700 Prague, Czech Republic.

International journal of molecular sciences

|June 28, 2023

概括

与心血管疾病相关的microRNA可以预测怀孕早期的流产或死产. 这项研究确定了用于早期检测的特定microRNA组合,可能有助于改善第一季度查.

科学领域:

生殖医学生殖医学
遗传学是一个遗传学.
生物标志物生物标志物

背景情况:

流产和死胎对怀孕的结果构成重大风险.
早期预测这些不良事件对于及时干预至关重要.
微RNA (miRNA) 正在成为各种产科并发症的潜在生物标志物.

研究的目的:

评估心血管疾病相关的microRNAs对于流产和死产的预测潜力.
为了识别特定的miRNA签名,用于早期检测流产.
评估在常规第一季度护理中实施基于miRNA的查的可行性.

主要方法:

在外周静脉血液样本中对29个microRNAs的基因表达进行了回顾性分析.
实时RT-PCR被用于量化miRNA水平.
样本来自单独的高加索怀孕,诊断出流产或死产和妊娠年龄匹配的对照.

主要成果:

在流产或死产的怀孕中观察到九种特定的微RNAs的改变表达.
一个由9个miRNA组成的小组实现了99.01%的准确性,预测妊娠流产的错误阳性率为10.0%.
使用特定miRNA组合的模型显示出对流产 (80.52%准确率) 和死产 (准确率高达95.83%) 的高预测能力.

结论:

与心血管疾病相关的microRNAs显示出高预测流产和死胎的潜力.
特定的miRNA组合可以用于早期和准确检测流产.
这些基于miRNA的模型可能是常规的第一季度查计划的宝贵补充.

关键词:

心血管疾病心血管疾病第一个三个月的查.基因表达的基因表达方式这是一个微型RNA.流产流产流产流产预测预测预测预测一个死产的死产.整个周围静脉血液的周围静脉血液

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...