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葡萄糖脱 - - 一种重要的水害虫对全球变暖的调节

Peng-Qi Quan¹, Jia-Rong Li¹, Xiang-Dong Liu¹

¹Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China.

International journal of molecular sciences

|June 28, 2023

概括

像大米叶文件这样的昆虫可以适应温度上升. 抑制葡萄糖脱酶基因 (CmGMC10) 有助于昆虫管理热应激和氧化损伤,提高生存率.

科学领域:

环境生物学环境生物学
昆虫生理学昆虫生理学
分子生物学分子生物学

背景情况:

全球变暖对动物群体,特别是昆虫构成重大威胁,因为它们容易受到热应激.
提高耐热性的昆虫适应机制尚未得到充分理解,这阻碍了对害虫对气候变化反应的预测.
米叶 (Cnaphalocrocis medinalis) 是一个主要的农业害虫,易受热应激的影响.

研究的目的:

为了研究大米叶文件中热适应的基础分子机制.
为了建立一种适应热量的昆虫菌株来研究耐热性.
确定特定基因在昆虫适应高温中的作用.

主要方法:

在连续几代的时间里,在39°C的温度下选择Cnaphalocrocis medinalis的第三阶段幼虫,以创建适应热量的菌株 (HA39).
在43°C的温度下,对HA39幼虫和未适应的菌株 (HA27) 的耐热性进行比较.
基因表达分析以确定分子变化,重点关注葡萄糖脱酶基因 (CmGMC10).
测量活性氧物种 (ROS) 和过氧化 (H₂O₂) 水平,以及抗氧化酶活性.

主要成果:

与HA27菌株相比,HA39菌株对急性热应激 (43°C) 的耐受性明显更高.
在HA39幼虫中观察到CmGMC10基因的升调,与ROS水平降低和存活率增加相关.
热适应幼虫在热应激下显示出增强的抗氧化酶活性和降低的H₂O₂水平,与CmGMC10表达有关.

结论:

叶幼虫的热度适应包括高调调 CmGMC10,一种葡萄糖脱酶基因.
这种基因上调增强了抗氧化酶活性,并减轻了热应激引起的氧化损伤,从而增加了生存率.
这些发现表明昆虫适应全球变暖的分子途径,突出显示CmGMC10是热耐受性的关键基因.

关键词:

这种形形 (Cnaphalocrocis medinalis) 是一个形形形.抗氧化酶可以帮助抗氧化酶.全球变暖全球变暖葡萄糖脱水酶的使用热度适应热度适应热度适应有活性氧物种的反应性氧物种.超氧化物脱酶的作用

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